{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:15:13Z","timestamp":1790511313808,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A peptide cocktail derived from the mycobacterial antigens ESAT-6, CFP-10, and Rv3615c allowed differentiation between<jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>-infected and M. bovis bacillus Calmette-Gu\u00e9rin (BCG)-vaccinated cattle when used as a skin test reagent for a \u201cDIVA\u201d test (i.e., a test capable of<jats:italic>d<\/jats:italic>ifferentiating<jats:italic>i<\/jats:italic>nfected and uninfected<jats:italic>v<\/jats:italic>accinated<jats:italic>a<\/jats:italic>nimals). Addition of the antigen Rv3020c improves the diagnostic sensitivity without compromising specificity in the face of BCG or Johne's disease vaccination.<\/jats:p>","DOI":"10.1128\/cvi.00024-12","type":"journal-article","created":{"date-parts":[[2012,2,2]],"date-time":"2012-02-02T19:54:14Z","timestamp":1328212454000},"page":"620-622","source":"Crossref","is-referenced-by-count":43,"title":["Improved Skin Test for Differential Diagnosis of Bovine Tuberculosis by the Addition of Rv3020c-Derived Peptides"],"prefix":"10.1128","volume":"19","author":[{"given":"Gareth J.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Whelan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Clifford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mick","family":"Coad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Martin","family":"Vordermeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2012,2,1]]},"reference":[{"key":"key20180919073603_B1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.vetmic.2008.09.060","article-title":"Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay","volume":"135","author":"Alvarez","year":"2009","journal-title":"Vet. Microbiol."},{"key":"key20180919073603_B2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.vetmic.2007.08.034","article-title":"Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection","volume":"128","author":"Alvarez","year":"2008","journal-title":"Vet. Microbiol."},{"key":"key20180919073603_B3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1051\/vetres:2006021","article-title":"Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis","volume":"37","author":"Aranaz","year":"2006","journal-title":"Vet. Res."},{"key":"key20180919073603_B4","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1126\/science.284.5419.1520","article-title":"Comparative genomics of BCG vaccines by whole-genome DNA microarray","volume":"284","author":"Behr","year":"1999","journal-title":"Science"},{"key":"key20180919073603_B5","unstructured":"DEFRA. 2010. The UK Chief Veterinary Officer's 2009 report on animal health and welfare. Department for Environment, Food and Rural Affairs, London, United Kingdom. http:\/\/www.defra.gov.uk\/corporate\/about\/who\/cvo\/documents\/2009report.pdf."},{"key":"key20180919073603_B6","doi-asserted-by":"crossref","first-page":"7877","DOI":"10.1073\/pnas.1130426100","article-title":"The complete genome sequence of Mycobacterium bovis","volume":"100","author":"Garnier","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919073603_B7","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1046\/j.1365-2958.1999.01383.x","article-title":"Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays","volume":"32","author":"Gordon","year":"1999","journal-title":"Mol. Microbiol."},{"key":"key20180919073603_B8","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1128\/CVI.00261-10","article-title":"Screening of predicted secreted antigens from Mycobacterium bovis identifies potential novel differential diagnostic reagents","volume":"17","author":"Jones","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073603_B9","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1046\/j.1439-0450.2001.00443.x","article-title":"Immune reactions in cattle after immunization with a Mycobacterium paratuberculosis vaccine and implications for the diagnosis of M. paratuberculosis and M. bovis infections","volume":"48","author":"Kohler","year":"2001","journal-title":"J. Vet. Med. B Infect. Dis. Vet. Public Health"},{"key":"key20180919073603_B10","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.178.5.1274-1282.1996","article-title":"Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis","volume":"178","author":"Mahairas","year":"1996","journal-title":"J. Bacteriol."},{"key":"key20180919073603_B11","doi-asserted-by":"crossref","first-page":"5730","DOI":"10.1073\/pnas.1015153108","article-title":"Rv3615c is a highly immunodominant RD1 (Region of Difference 1)-dependent secreted antigen specific for Mycobacterium tuberculosis infection","volume":"108","author":"Millington","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"key20180919073603_B12","doi-asserted-by":"crossref","first-page":"5628","DOI":"10.1128\/IAI.73.9.5628-5635.2005","article-title":"Bovine NK cells can produce gamma interferon in response to the secreted mycobacterial proteins ESAT-6 and MPP14 but not in response to MPB70","volume":"73","author":"Olsen","year":"2005","journal-title":"Infect. Immun."},{"key":"key20180919073603_B13","doi-asserted-by":"crossref","first-page":"3932","DOI":"10.1128\/IAI.00150-08","article-title":"Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosis complex","volume":"76","author":"Sidders","year":"2008","journal-title":"Infect. Immun."},{"key":"key20180919073603_B14","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1128\/IAI.73.7.4441-4444.2005","article-title":"The order of prime-boost vaccination of neonatal calves with Mycobacterium bovis BCG and a DNA vaccine encoding mycobacterial proteins Hsp65, Hsp70, and Apa is not critical for enhancing protection against bovine tuberculosis","volume":"73","author":"Skinner","year":"2005","journal-title":"Infect. Immun."},{"key":"key20180919073603_B15","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1128\/CDLI.6.5.675-682.1999","article-title":"Development of diagnostic reagents to differentiate between Mycobacterium bovis BCG vaccination and M. bovis infection in cattle","volume":"6","author":"Vordermeier","year":"1999","journal-title":"Clin. Diagn. Lab Immunol."},{"key":"key20180919073603_B16","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1128\/IAI.74.2.1416-1418.2006","article-title":"Immune responses induced in cattle by vaccination with a recombinant adenovirus expressing mycobacterial antigen 85A and Mycobacterium bovis BCG","volume":"74","author":"Vordermeier","year":"2006","journal-title":"Infect. Immun."},{"key":"key20180919073603_B17","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1365-2567.2004.01903.x","article-title":"Cellular immune responses induced in cattle by heterologous prime-boost vaccination using recombinant viruses and bacille Calmette-Guerin","volume":"112","author":"Vordermeier","year":"2004","journal-title":"Immunology"},{"key":"key20180919073603_B18","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1128\/IAI.00287-09","article-title":"Viral booster vaccines improve Mycobacterium bovis BCG-induced protection against bovine tuberculosis","volume":"77","author":"Vordermeier","year":"2009","journal-title":"Infect. Immun."},{"key":"key20180919073603_B19","doi-asserted-by":"crossref","first-page":"3540","DOI":"10.1128\/IAI.73.6.3540-3546.2005","article-title":"Vaccination of cattle with a CpG oligodeoxynucleotide-formulated mycobacterial protein vaccine and Mycobacterium bovis BCG induces levels of protection against bovine tuberculosis superior to those induced by vaccination with BCG alone","volume":"73","author":"Wedlock","year":"2005","journal-title":"Infect. Immun."},{"key":"key20180919073603_B20","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.00420-10","article-title":"Development of a skin test for bovine tuberculosis for differentiating infected from vaccinated animals","volume":"48","author":"Whelan","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"key20180919073603_B21","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1751-0813.1991.tb03254.x","article-title":"Field comparison of the interferon-gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis","volume":"68","author":"Wood","year":"1991","journal-title":"Aust. Vet. J."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00024-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:46:50Z","timestamp":1627591610000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00024-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,1]]},"references-count":21,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2012,3,28]]},"published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1128\/CVI.00024-12"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00024-12","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,1]]}}}