{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:15:35Z","timestamp":1747188935189,"version":"3.40.5"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Serology has a pivotal role in the diagnosis of congenital syphilis (CS), but problems arise because of the passive transfer of IgG antibodies across the placenta. The aim of this study was to assess the diagnostic value of a comparative Western blot (WB) method finalized to match the IgG immunological profiles of mothers and their own babies at birth in order to differentiate between passively transmitted maternal antibodies and antibodies synthesized by the infants against<jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>. Thirty infants born to mothers with unknown or inadequate treatment for syphilis were entered in a retrospective study, conducted at St. Orsola-Malpighi Hospital, Bologna, Italy. All of the infants underwent clinical, instrumental, and laboratory examinations, including IgM WB testing. For the retrospective study, an IgG WB assay was performed by blotting<jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>antigens onto nitrocellulose sheets and incubating the strips with serum specimens from mother-child pairs. CS was diagnosed in 11 out of the 30 enrolled infants; 9\/11 cases received the definitive diagnosis within the first week of life, whereas the remaining two were diagnosed later because of increasing serological test titers. The use of the comparative IgG WB testing performed with serum samples from mother-child pairs allowed a correct CS diagnosis in 10\/11 cases. The CS diagnosis was improved by a strategy combining comparative IgG WB results with IgM WB results, leading to a sensitivity of 100%. The comparative IgG WB test is thus a welcome addition to the conventional laboratory methods used for CS diagnosis, allowing identification and adequate treatment of infected infants and avoiding unnecessary therapy of uninfected newborns.<\/jats:p>","DOI":"10.1128\/cvi.00032-16","type":"journal-article","created":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T03:48:44Z","timestamp":1457581724000},"page":"410-416","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Contribution of a Comparative Western Blot Method to Early Postnatal Diagnosis of Congenital Syphilis"],"prefix":"10.1128","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7397-6560","authenticated-orcid":false,"given":"Antonella","family":"Marangoni","sequence":"first","affiliation":[{"name":"DIMES, Microbiology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Foschi","sequence":"additional","affiliation":[{"name":"DIMES, Microbiology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Capretti","sequence":"additional","affiliation":[{"name":"Department of Obstetrical, Gynaecological and Paediatric Sciences, Operative Unit of Neonatology, St. Orsola-Malpighi Hospital, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Nardini","sequence":"additional","affiliation":[{"name":"DIMES, Microbiology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Compri","sequence":"additional","affiliation":[{"name":"DIMES, Microbiology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi Tommaso","family":"Corvaglia","sequence":"additional","affiliation":[{"name":"DIMEC, Neonatology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giacomo","family":"Faldella","sequence":"additional","affiliation":[{"name":"DIMEC, Neonatology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Cevenini","sequence":"additional","affiliation":[{"name":"DIMES, Microbiology, University of Bologna, Bologna, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70104-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001396"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.12.107623"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02066.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31826ac25f"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462415580226"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2013.08.005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"23A","DOI":"10.1155\/2015\/589085","article-title":"Canadian Public Health Laboratory Network laboratory guidelines for congenital syphilis and syphilis screening in pregnant women in Canada","volume":"26","author":"Singh AE","year":"2015","unstructured":"Singh AE, Levett PN, Fonseca K, Jayaraman GC, Lee BE. 2015. 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