{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:55:25Z","timestamp":1787838925338,"version":"build-2784847793"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>One alternative for management of brucellosis in Yellowstone National Park bison (<jats:italic>Bison bison<\/jats:italic>) is vaccination of calves and yearlings. Although<jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>strain RB51 vaccination protects bison against experimental challenge, the effect of booster vaccinations was unknown. This study characterized immunologic responses after dart or booster vaccination of bison with<jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>strain RB51. In two studies, 8- to 10-month-old female bison were inoculated with saline (<jats:italic>n<\/jats:italic>= 14), hand vaccinated with 1.1 \u00d7 10<jats:sup>10<\/jats:sup>to 2.0 \u00d7 10<jats:sup>10<\/jats:sup>CFU of RB51 (<jats:italic>n<\/jats:italic>= 21), or dart vaccinated with 1.8 \u00d7 10<jats:sup>10<\/jats:sup>CFU of RB51 (<jats:italic>n<\/jats:italic>= 7). A subgroup of hand vaccinates in study 1 was randomly selected for booster vaccination 15 months later with 2.2 \u00d7 10<jats:sup>10<\/jats:sup>CFU of RB51. Compared to single vaccinates, booster-vaccinated bison had greater serologic responses to RB51. However, there was a trend for antigen-specific proliferative responses of peripheral blood mononuclear cells (PBMC) from booster vaccinates to be reduced compared to responses of PBMC from single vaccinates. PBMC from booster vaccinates tended to have greater gamma interferon (IFN-\u03b3) production than those from single vaccinates. In general, dart vaccination with RB51 induced immunologic responses similar to those of hand vaccination. All vaccinates (single hand, dart, or booster) demonstrated greater (<jats:italic>P<\/jats:italic>&lt; 0.05) immunologic responses at various times after vaccination than nonvaccinated bison. Booster vaccination with RB51 in early gestation did not induce abortion or fetal infection. Our data suggest that booster vaccination does not induce strong anamnestic responses. However, phenotypic data on resistance to experimental challenge are required to fully assess the effect of booster vaccination on protective immunity.<\/jats:p>","DOI":"10.1128\/cvi.00033-12","type":"journal-article","created":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T05:45:05Z","timestamp":1333086305000},"page":"642-648","source":"Crossref","is-referenced-by-count":8,"title":["Immune Responses and Safety after Dart or Booster Vaccination of Bison with Brucella abortus Strain RB51"],"prefix":"10.1128","volume":"19","author":[{"given":"S. C.","family":"Olsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"key20180919073603_B1","first-page":"17","article-title":"Techniques for the brucellosis laboratory","volume-title":"Institut National de la Recherche Agronomique","author":"Alton","year":"1988"},{"key":"key20180919073603_B2"},{"key":"key20180919073603_B3","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/j.1348-0421.2009.00141.x","article-title":"Study of the immunologic profile towards Mycobacterium bovis antigens in naturally infected cattle","volume":"53","author":"Blanco","year":"2009","journal-title":"Microbiol. Immunol."},{"key":"key20180919073603_B4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.vetimm.2005.08.002","article-title":"Identification of immune response correlates for protection against bovine tuberculosis","volume":"108","author":"Buddle","year":"2005","journal-title":"Vet. Immunol. Immunopathol."},{"key":"key20180919073603_B5","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.2460\/ajvr.1996.57.08.1153","article-title":"Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis","volume":"57","author":"Cheville","year":"1996","journal-title":"Am. J. Vet. Res."},{"key":"key20180919073603_B6","doi-asserted-by":"crossref","first-page":"360","DOI":"10.7589\/0090-3558-26.3.360","article-title":"Brucella abortus in captive bison. I. Serology, bacteriology, pathogenesis and transmission to cattle","volume":"26","author":"Davis","year":"1990","journal-title":"J. Wildl. Dis."},{"key":"key20180919073603_B7","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.vetimm.2008.05.019","article-title":"Antigen-specific regulatory T cells in bovine paratuberculosis","volume":"125","author":"De Almeida","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"key20180919073603_B8","first-page":"223","article-title":"Effects of exogenous recombinant interleukin-12 on immune responses and protection against Brucella abortus in a murine model","volume":"65","author":"Lee","year":"2001","journal-title":"Can. J. Vet. Res."},{"key":"key20180919073603_B9","first-page":"105","article-title":"Annual report of the Bureau of Animal Industry","volume-title":"Department of Agriculture","author":"Mohler","year":"1917"},{"key":"key20180919073603_B10","first-page":"183","article-title":"Immune responses and efficacy after administration of a commercial Brucella abortus strain RB51 vaccine to cattle","volume":"1","author":"Olsen","year":"2000","journal-title":"Vet. Ther."},{"key":"key20180919073603_B11","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/CVI.00419-08","article-title":"Immune responses and protection against experimental challenge after vaccination of bison with Brucella abortus strain RB51 or RB51 overexpressing superoxide dismutase and glycosyltransferase genes","volume":"16","author":"Olsen","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073603_B12","doi-asserted-by":"crossref","first-page":"738","DOI":"10.7589\/0090-3558-38.4.738","article-title":"Immune responses of bison to ballistic or hand vaccination with Brucella abortus strain RB51","volume":"38","author":"Olsen","year":"2002","journal-title":"J. Wildl. Dis."},{"key":"key20180919073603_B13","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"Pfaffl","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"key20180919073603_B14","first-page":"147","article-title":"Study of the gene expression of Th1 and Th2 cytokines in the immune response of cows vaccinated with Brucella abortus RB51","volume":"42","author":"Polci","year":"2006","journal-title":"Vet. Ital."},{"key":"key20180919073603_B15","doi-asserted-by":"crossref","first-page":"950","DOI":"10.2307\/3802809","article-title":"Biosafety of parenteral Brucella abortus RB51 vaccine in bison calves","volume":"63","author":"Roffe","year":"1999","journal-title":"J. Wildl. Manag."},{"key":"key20180919073603_B16","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1128\/CVI.05201-11","article-title":"Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease","volume":"18","author":"Shu","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"key20180919073603_B17","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.vetimm.2007.05.009","article-title":"Associations between cytokine gene expression and pathology in Mycobacterium bovis infected cattle","volume":"119","author":"Thacker","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"key":"key20180919073603_B18","unstructured":"U.S. Department of the Interior and Department of Agriculture. 2000. Final environmental impact statement and bison management plan for the state of Montana and Yellowstone National Park, p vii-viii, 282\u2013283. www.nps.gov\/yell\/parkmgmt\/upload\/yellbisonrod.pdf."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00033-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T11:21:13Z","timestamp":1713784873000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00033-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,29]]},"references-count":18,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2012,4,26]]},"published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1128\/CVI.00033-12"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00033-12","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,29]]}}}