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To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples from<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>smooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-\u03b3) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-\u03b3 ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-\u03b3. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-\u03b3 recovery over 10 days when stored at room temperature.<\/jats:p>","DOI":"10.1128\/cvi.00033-13","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T03:42:52Z","timestamp":1377747772000},"page":"1669-1674","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon"],"prefix":"10.1128","volume":"20","author":[{"given":"Lucy","family":"Duncombe","sequence":"first","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola J.","family":"Commander","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sevil","family":"Erdenlig","sequence":"additional","affiliation":[{"name":"Pendik Veterinary Control and Research Institute, Pendik, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"McGiven","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Stack","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco MP","year":"2007","unstructured":"FrancoMPMulderMGilmanRHSmitsHL. 2007. 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