{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:10:53Z","timestamp":1770754253242,"version":"3.50.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Toward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents an M3 epitope similar to that of<jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>, was evaluated as a vaccine candidate in the hamster model of leptospirosis. LemA is conserved (&gt;70% pairwise identity) among the pathogenic<jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>spp., indicating its potential in stimulating a cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and a subunit preparation, recombinant LemA conferred different levels of protection in hamsters. Significant protection against mortality was observed for the prime-boost and the DNA vaccine strategies, which showed 87.5% (<jats:italic>P<\/jats:italic>&lt; 0.01) and 62.5% (<jats:italic>P<\/jats:italic>&lt; 0.05) efficacy, respectively. Although the subunit vaccine preparation protected 50.0% of immunized hamsters, the level of protection was not significant. None of the hamsters in the control groups survived challenge with a virulent strain of<jats:named-content content-type=\"genus-species\">Leptospira interrogans<\/jats:named-content>serogroup Icterohaemorrhagiae. Characterization of the immune response found that the strongest antibody response was stimulated by the subunit vaccine preparation, followed by the prime-boost strategy. The DNA vaccine failed to elicit an antibody response in immunized hamsters.<\/jats:p>","DOI":"10.1128\/cvi.00034-13","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T05:11:45Z","timestamp":1363842705000},"page":"747-752","source":"Crossref","is-referenced-by-count":38,"title":["A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis"],"prefix":"10.1128","volume":"20","author":[{"given":"Daiane D.","family":"Hartwig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine M.","family":"Forster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds L.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J. 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