{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:48:18Z","timestamp":1789444098232,"version":"build-2803163510"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Individuals with defects in T cell-mediated immunity (CMI) are highly susceptible to infection with<jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>. The purpose of these studies was to determine if protection against experimental pulmonary cryptococcosis can be generated in T cell-deficient hosts. BALB\/c mice were depleted of CD4<jats:sup>+<\/jats:sup>and\/or CD8<jats:sup>+<\/jats:sup>T cells or given an isotype control antibody prior to vaccination with a<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>strain, designated H99\u03b3, previously shown to induce protection against<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>infection in immunocompetent mice. Mice depleted of CD4<jats:sup>+<\/jats:sup>or CD8<jats:sup>+<\/jats:sup>T cells, but not both subsets, survived an acute pulmonary infection with<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>strain H99\u03b3 and a subsequent second challenge with wild-type<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>strain H99. We observed a significant increase in the percentage of CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cells expressing the activation marker CD69 in the lungs of mice immunized with<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>strain H99\u03b3 prior to a secondary challenge with wild-type cryptococci. CD4<jats:sup>+<\/jats:sup>T cells within the lungs of immunized mice also appeared to acquire a predominantly activated effector memory cell phenotype (CD69<jats:sup>+<\/jats:sup>CD44<jats:sup>+<\/jats:sup>CCR7<jats:sup>\u2212<\/jats:sup>CD45RB<jats:sup>\u2212<\/jats:sup>CD62L<jats:sup>\u2212<\/jats:sup>) following a second pulmonary challenge with wild-type<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>, compared to CD4<jats:sup>+<\/jats:sup>T cells from na\u00efve mice. Lastly, immunization of immunocompetent mice with<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>strain H99\u03b3 prior to depletion of CD4<jats:sup>+<\/jats:sup>and\/or CD8<jats:sup>+<\/jats:sup>T cells resulted in significant protection against a second challenge with wild-type<jats:named-content content-type=\"genus-species\">C. neoformans<\/jats:named-content>. Our studies demonstrate that protective immunity against pulmonary cryptococcosis can be generated in immunosuppressed hosts, thus supporting the development of cryptococcal vaccines.<\/jats:p>","DOI":"10.1128\/cvi.00036-11","type":"journal-article","created":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T03:40:14Z","timestamp":1301542814000},"page":"717-723","source":"Crossref","is-referenced-by-count":64,"title":["Protective Immunity against Experimental Pulmonary Cryptococcosis in T Cell-Depleted Mice"],"prefix":"10.1128","volume":"18","author":[{"given":"Karen L.","family":"Wozniak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattie L.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Floyd L.","family":"Wormley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2011,3,30]]},"reference":[{"key":"key20180919073511_B1","doi-asserted-by":"crossref","first-page":"3173","DOI":"10.1002\/eji.200526004","article-title":"Novel phenotypes and migratory properties distinguish memory CD4 T cell subsets in lymphoid and lung tissue","volume":"35","author":"Bingaman","year":"2005","journal-title":"Eur. 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