{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:18:34Z","timestamp":1761675514650},"reference-count":34,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Previous studies have identified the length dependency of several polysaccharide (PS) protective epitopes. We have investigated whether meningococcal polysaccharides Y and W-135 possess such epitopes. Oligosaccharides (OSs) consisting of one or more disaccharide repeating units (RU) were derived from the capsular PSs of group Y and W-135 meningococci (GYMP and GWMP, respectively) by mild acid hydrolysis. The relative affinities of anticapsular antibodies binding to derivative OSs of different chain lengths were measured in inhibition enzyme-linked immunosorbent assays. As OS size increased from two to three RU, there was a notable increase in binding inhibition of rabbit anti-group Y antiserum. This pattern of antibody binding inhibition was also observed for rabbit antiserum to group W-135, though the inhibition increase was much more pronounced. In the cases of both OS species, the concentration of inhibiting antigen required to achieve 50% inhibition of rabbit immunoglobulin binding increased progressively as the inhibiting disaccharide chain length increased from 1 RU through greater than 50 RU. These data suggest that antibodies directed against both of these meningococcal PSs recognize conformational epitopes only fully expressed in higher-molecular-weight forms of these antigens.<\/jats:p>","DOI":"10.1128\/cvi.00049-07","type":"journal-article","created":{"date-parts":[[2007,9,6]],"date-time":"2007-09-06T01:26:24Z","timestamp":1189041984000},"page":"1311-1317","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Epitope Specificities of the Group Y and W-135 Polysaccharides of<i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"14","author":[{"given":"Samuel L.","family":"Moore","sequence":"first","affiliation":[{"name":"Wellstat Vaccines, 9 West Watkins Mill Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Uitz","sequence":"additional","affiliation":[{"name":"Emergent Biosolutions, 300 Professional Drive, Gaithersburg, Maryland 20879"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Chun","family":"Ling","sequence":"additional","affiliation":[{"name":"Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Bundle","sequence":"additional","affiliation":[{"name":"Alberta Ingenuity Centre for Carbohydrate Science, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Fusco","sequence":"additional","affiliation":[{"name":"Wellstat Vaccines, 9 West Watkins Mill Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Michon","sequence":"additional","affiliation":[{"name":"Wellstat Vaccines, 9 West Watkins Mill Road, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1016\/j.vaccine.2004.10.021","volume":"23","year":"2005","unstructured":"Bardotti, A., G. 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