{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:13:10Z","timestamp":1787627590999,"version":"build-2736575974"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The development of safe and efficient avian influenza vaccines for human and animal uses is essential for preventing virulent outbreaks and pandemics worldwide. In this study, we constructed a recombinant (<jats:named-content content-type=\"genus-species\">pgsA<\/jats:named-content>-HA1 gene fusion)<jats:named-content content-type=\"genus-species\">Lactococcus lactis<\/jats:named-content>strain that expresses and displays the avian influenza virus HA1 antigens on its surface. The vectors were administered by oral delivery with or without the addition of cholera toxin subunit B (CTB). The resulting immune responses were analyzed, and the mice were eventually challenged with lethal doses of H5N1 viruses. Significant titers of hemagglutinin (HA)-specific serum IgG and fecal IgA were detected in the group that also received CTB. Cellular immunities were also shown in both cell proliferation and gamma interferon (IFN-\u03b3) enzyme-linked immunospot (ELISpot) assays. Most importantly, the mice that received the<jats:named-content content-type=\"genus-species\">L. lactis<\/jats:named-content><jats:named-content content-type=\"genus-species\">pgsA<\/jats:named-content>-HA1 strain combined with CTB were completely protected from lethal challenge of the H5N1 virus. These findings support the further development of<jats:named-content content-type=\"genus-species\">L. lactis<\/jats:named-content>-based avian influenza virus vaccines for human and animal uses.<\/jats:p>","DOI":"10.1128\/cvi.00050-11","type":"journal-article","created":{"date-parts":[[2011,6,3]],"date-time":"2011-06-03T05:54:00Z","timestamp":1307080440000},"page":"1046-1051","source":"Crossref","is-referenced-by-count":50,"title":["Evaluation of Oral Immunization with Recombinant Avian Influenza Virus HA1 Displayed on the Lactococcus lactis Surface and Combined with the Mucosal Adjuvant Cholera Toxin Subunit B"],"prefix":"10.1128","volume":"18","author":[{"given":"Han","family":"Lei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhina","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic Man-Kit","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2011,6,1]]},"reference":[{"key":"key20180919073519_B1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/351479a0","article-title":"Humoral and cell-mediated immune response to live recombinant BCG-HIV vaccines","volume":"351","author":"Aldovini","year":"1991","journal-title":"Nature"},{"key":"key20180919073519_B2","doi-asserted-by":"crossref","first-page":"2706","DOI":"10.1016\/j.micinf.2006.07.018","article-title":"Intranasal administration of adjuvant-combined recombinant influenza virus HA vaccine protects mice from the lethal H5N1 virus infection","volume":"8","author":"Asahi-Ozaki","year":"2006","journal-title":"Microbes Infect"},{"key":"key20180919073519_B3","doi-asserted-by":"crossref","first-page":"5304","DOI":"10.1016\/j.vaccine.2008.07.047","article-title":"Lactococcus lactis-expressing listeriolysin O (LLO) provides protection and specific CD8+ T cells against Listeria monocytogenes in the murine infection model","volume":"26","author":"Bahey-El-Din","year":"2008","journal-title":"Vaccine"},{"key":"key20180919073519_B4","doi-asserted-by":"crossref","first-page":"264","DOI":"10.4161\/hv.5.4.7553","article-title":"Lactococcus lactis as a live vector for mucosal delivery of therapeutic proteins","volume":"504","author":"Berm\u00fadez-Humar\u00e1n","year":"2009","journal-title":"Hum. 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