{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T21:03:12Z","timestamp":1781989392178,"version":"3.54.5"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Brucellosis is a serious zoonosis that occurs worldwide, and its diagnosis is typically based on the detection of antibodies against\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            lipopolysaccharide (LPS). However, the specificity of the LPS-based test is compromised by cross-reactivity with\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            O157:H7 and\n            <jats:named-content content-type=\"genus-species\">Yersinia enterocolitica<\/jats:named-content>\n            O:9. Also, diagnosis based on the LPS test cannot differentiate between vaccinated and infected individuals. The detection of the 26-kDa cytosoluble protein (BP26) antibody is considered an alternative that circumvents these drawbacks because it is exclusively expressed by infectious\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            . A BP26-based enzyme-linked immunosorbent assay (ELISA) has been tried for the diagnosis of\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            -infected animals and humans, but a few results showed that BP26 couldn't react with all\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            -positive sera. In order to explore whether different animals could produce antibodies against BP26 after being infected with various\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species, we infected sheep, goats, and beef cattle with common virulent reference\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species. All sera were collected from the experimental animals and tested using both LPS-based ELISAs and BP26-based ELISAs. The results showed that all\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            -infected individuals could produce high levels of antibodies against LPS, but only\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            16M- and\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            M28-infected sheep and\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            16M- and\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            2308-infected goats could produce antibodies against BP26. Therefore, we concluded that the BP26-based indirect ELISA (i-ELISA) showed both\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species and host specificity, which obviously limits its reliability as a substitute for the traditional LPS-based ELISA for the detection of brucellosis.\n          <\/jats:p>","DOI":"10.1128\/cvi.00052-13","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T03:58:20Z","timestamp":1374119900000},"page":"1410-1417","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Limitations of the BP26 Protein-Based Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis"],"prefix":"10.1128","volume":"20","author":[{"given":"Ting","family":"Xin","sequence":"first","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjun","family":"Yang","sequence":"additional","affiliation":[{"name":"Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University, Taian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiguang","family":"Wang","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairong","family":"Mao","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabo","family":"Ding","sequence":"additional","affiliation":[{"name":"China Institute of Veterinary Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.2217\/fmb.11.136","article-title":"Brucellosis and type IV secretion","volume":"7","author":"de Jongand MF","year":"2012","unstructured":"de JongandMFTsolisRM. 2012. 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