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In this study, the B domains of a group G<jats:named-content content-type=\"genus-species\">Streptococcus<\/jats:named-content>(SpG) strain and<jats:named-content content-type=\"genus-species\">Peptostreptococcus magnus<\/jats:named-content>(PpL) were used to evaluate whether vaccine efficacy is influenced by the type of immunoglobulin-binding domain (IBD) in the tricomponent complex. IBDs were fused to a pentameric cartilage oligomeric matrix protein (COMP) to increase the binding avidity of the complexes for their targets. The COMP-IBD fusion proteins generated (COMP-SpG and COMP-PpL and the previously constructed COMP-Z) bound a large fraction of splenic B lymphocytes but not T lymphocytes. These carrier molecules were then loaded with an ookinete surface protein of<jats:named-content content-type=\"genus-species\">Plasmodium vivax<\/jats:named-content>, Pvs25, by chemical conjugation. The administration of the tricomponent complexes to mice induced more Pvs25-specific serum IgG than did the unloaded antigen. The PpL complex, which exhibited a broad Ig-binding spectrum, conferred higher vaccine efficacy than did the Z or SpG complexes when evaluated with a membrane feed assay. This study demonstrates that this tricomponent immunopotentiating system, incorporating IBDs as the B-lymphocyte-targeting ligands, is a promising technology for the delivery of malaria vaccines, particularly when combined with an aluminum salt adjuvant.<\/jats:p>","DOI":"10.1128\/cvi.00053-14","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T06:02:01Z","timestamp":1392271321000},"page":"561-569","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Tricomponent Complex Loaded with a Mosquito-Stage Antigen of the Malaria Parasite Induces Potent Transmission-Blocking Immunity"],"prefix":"10.1128","volume":"21","author":[{"given":"Takeshi","family":"Arakawa","sequence":"first","affiliation":[{"name":"Molecular Microbiology Group, Department of Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Ehime, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jetsumon","family":"Sattabongkot","sequence":"additional","affiliation":[{"name":"Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kozue","family":"Sakao","sequence":"additional","affiliation":[{"name":"Division of Molecular Functions of Food, Department of Biochemistry and Biotechnology, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motomi","family":"Torii","sequence":"additional","affiliation":[{"name":"Division of Molecular Parasitology, Proteo-Science Center, Ehime University, Toon, Ehime, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Miyata","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Group, Department of Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.30875\/ac42f7b8-en"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3077"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.64.97"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/333074a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(90)90096-9"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6618-6623.2000"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5769(02)00037-5"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00258-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7375-7380.2005"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00306-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.01.083"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05214-11"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.12.019"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002636"},{"key":"e_1_3_2_16_2","volume-title":"Tables of malaria vaccine projects globally. 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