{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:04:03Z","timestamp":1771661043103,"version":"3.50.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>can cause serious public health problems and economic losses worldwide. Calcium-dependent protein kinases (CDPKs) are key mediators of<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>signaling pathways and are implicated as important virulence factors. In the present study, we cloned a novel<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>CDPK gene, named TgCDPK5, and constructed the eukaryotic expression vector pVAX-CDPK5. Then, we evaluated the immune protection induced by pVAX-CDPK5 in Kunming mice. After injection of pVAX-CDPK5 intramuscularly, immune responses, determined with lymphoproliferative assays and cytokine and antibody measurements, were monitored, and mouse survival times and brain cyst formation were evaluated following challenges with the<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>RH strain (genotype I) and the PRU strain (genotype II). pVAX-CDPK5 effectively induced immune responses with increased specific antibodies, a predominance of IgG2a production, and a strong lymphocyte proliferative response. The levels of gamma interferon (IFN-\u03b3), interleukin 2 (IL-2), and IL-12(p70) and the percentages of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>and CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>cells in mice vaccinated with pVAX-CDPK5 were significantly increased. However, IL-4 and IL-10 were not produced in the vaccinated mice. These results demonstrate that pVAX-CDPK5 can elicit strong humoral and cellular Th1 immune responses. The survival time of immunized mice challenged with the<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>RH strain (8.67 \u00b1 4.34 days) was slightly, but not significantly, longer than that in the control groups within 7 days (<jats:italic>P<\/jats:italic>&gt; 0.05). The numbers of brain cysts in the mice in the pVAX-CDPK5 group were reduced by \u223c40% compared with those in the control groups (<jats:italic>P<\/jats:italic>&lt; 0.05), which provides a foundation for the further development of effective subunit vaccines against<jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/cvi.00059-14","type":"journal-article","created":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T10:16:46Z","timestamp":1398939406000},"page":"924-929","source":"Crossref","is-referenced-by-count":24,"title":["Evaluation of Immune Responses in Mice after DNA Immunization with Putative Toxoplasma gondii Calcium-Dependent Protein Kinase 5"],"prefix":"10.1128","volume":"21","author":[{"given":"Nian-Zhang","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si-Yang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Peng","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2014,4,30]]},"reference":[{"key":"key20180919073737_B1","first-page":"1","volume-title":"Toxoplasmosis of animals and humans","author":"Dubey","year":"2010","edition":"2nd ed"},{"key":"key20180919073737_B2","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin. 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