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Initially, we isolated the homolog genes encoding four<jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>.)<jats:italic>braziliensis<\/jats:italic>antigens: (i) homologue of receptor for activated C kinase, (ii) thiol-specific antioxidant, (iii)<jats:italic>Leishmania<\/jats:italic>elongation and initiation factor, and (iv)<jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.)<jats:italic>major<\/jats:italic>stress-inducible protein 1. At the deduced amino acid level, all four open reading frames had a high degree of identity with the previously described genes of<jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.)<jats:italic>major<\/jats:italic>being expressed on promastigotes and amastigotes of<jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>.)<jats:italic>braziliensis<\/jats:italic>. These genes were inserted into the vector pcDNA3 or expressed as bacterial recombinant proteins. After immunization with recombinant plasmids or proteins, BALB\/c mice generated specific antibody or cell-mediated immune responses (gamma interferon production). After an intradermal challenge with<jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>.)<jats:italic>braziliensis<\/jats:italic>infective promastigotes, no significant reduction on the lesions was detected. We conclude that the protective immunity afforded by these four vaccine candidates against experimental cutaneous leishmaniasis caused by<jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.)<jats:italic>major<\/jats:italic>could not be reproduced against a challenge with<jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>.)<jats:italic>braziliensis<\/jats:italic>. Although negative, we consider our results important since they suggest that studies aimed at the development of an effective vaccine against<jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>.)<jats:italic>braziliensis<\/jats:italic>, the main causative agent of cutaneous leishmaniasis in the New World, should be redirected toward distinct antigens or different vaccination strategies.<\/jats:p>","DOI":"10.1128\/cvi.00060-07","type":"journal-article","created":{"date-parts":[[2007,7,12]],"date-time":"2007-07-12T01:04:26Z","timestamp":1184202266000},"page":"1173-1181","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Testing of Four<i>Leishmania<\/i>Vaccine Candidates in a Mouse Model of Infection with<i>Leishmania<\/i>(<i>Viannia<\/i>)<i>braziliensis<\/i>, the Main Causative Agent of Cutaneous Leishmaniasis in the New World"],"prefix":"10.1128","volume":"14","author":[{"given":"G.","family":"Salay","sequence":"first","affiliation":[{"name":"Centro Interdisciplinar de Terapia Ge\u0302nica, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Mirassol, 207, Sa\u0303o Paulo, SP 04044-010, Brazil"},{"name":"Disciplina de Parasitologia-Departamento de Microbiologia, Imunologiae Parasitologia, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Botucatu, 862, 60 Andar, Sa\u0303o Paulo, SP 04023-062, Brazil"},{"name":"Disciplina de Imunologia-Departamento de Microbiologia Imunonologia e Parasitologia, Universidade Federal de Goia\u0302nia, Goia\u0302nia, Brazil"},{"name":"Centro de Pesquisas Gonc\u0327alo Moniz, Fundac\u0327a\u0303o Oswaldo Cruz, Rua Waldemar Falca\u0303o, 121, Salvador, BA 40295-001, Brazil"},{"name":"Departamento de Biointerac\u0327a\u0303o, Instituto de Cie\u0302ncias da Sau\u0301de, Universidade Federal da Bahia, Av. 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L.","family":"Dorta","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Terapia Ge\u0302nica, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Mirassol, 207, Sa\u0303o Paulo, SP 04044-010, Brazil"},{"name":"Disciplina de Parasitologia-Departamento de Microbiologia, Imunologiae Parasitologia, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Botucatu, 862, 60 Andar, Sa\u0303o Paulo, SP 04023-062, Brazil"},{"name":"Disciplina de Imunologia-Departamento de Microbiologia Imunonologia e Parasitologia, Universidade Federal de Goia\u0302nia, Goia\u0302nia, Brazil"},{"name":"Centro de Pesquisas Gonc\u0327alo Moniz, Fundac\u0327a\u0303o Oswaldo Cruz, Rua Waldemar Falca\u0303o, 121, Salvador, BA 40295-001, Brazil"},{"name":"Departamento de Biointerac\u0327a\u0303o, Instituto de Cie\u0302ncias da Sau\u0301de, Universidade Federal da Bahia, Av. 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M.","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Terapia Ge\u0302nica, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Mirassol, 207, Sa\u0303o Paulo, SP 04044-010, Brazil"},{"name":"Disciplina de Parasitologia-Departamento de Microbiologia, Imunologiae Parasitologia, Universidade Federal de Sa\u0303o Paulo-Escola Paulista de Medicina, Rua Botucatu, 862, 60 Andar, Sa\u0303o Paulo, SP 04023-062, Brazil"},{"name":"Disciplina de Imunologia-Departamento de Microbiologia Imunonologia e Parasitologia, Universidade Federal de Goia\u0302nia, Goia\u0302nia, Brazil"},{"name":"Centro de Pesquisas Gonc\u0327alo Moniz, Fundac\u0327a\u0303o Oswaldo Cruz, Rua Waldemar Falca\u0303o, 121, Salvador, BA 40295-001, Brazil"},{"name":"Departamento de Biointerac\u0327a\u0303o, Instituto de Cie\u0302ncias da Sau\u0301de, Universidade Federal da Bahia, Av. 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