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These results demonstrate that care must be taken to ensure that LF preparations used in standardized cell-based assays are not altered at their N-terminal ends.<\/jats:p>","DOI":"10.1128\/cvi.00081-08","type":"journal-article","created":{"date-parts":[[2008,9,25]],"date-time":"2008-09-25T00:44:55Z","timestamp":1222303495000},"page":"1737-1741","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Role of the N-Terminal Amino Acid of\n            <i>Bacillus anthracis<\/i>\n            Lethal Factor in Lethal Toxin Cytotoxicity and Its Effect on the Lethal Toxin Neutralization Assay"],"prefix":"10.1128","volume":"15","author":[{"given":"Anita","family":"Verma","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Wagner","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Stibitz","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nga","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flor","family":"Guerengomba","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drusilla L.","family":"Burns","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.3133-3138.2005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1126\/science.3018930","volume":"234","year":"1986","unstructured":"Bachmair, A., D. 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