{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T03:12:21Z","timestamp":1776913941226,"version":"3.51.2"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Baylisascaris<\/jats:named-content>larva migrans is an important zoonotic disease caused by<jats:named-content content-type=\"genus-species\">Baylisascaris procyonis<\/jats:named-content>, the raccoon roundworm, and is being increasingly considered in the differential diagnosis of eosinophilic meningoencephalitis in children and young adults. Although a<jats:named-content content-type=\"genus-species\">B. procyonis<\/jats:named-content>excretory-secretory (BPES) antigen-based enzyme-linked immunosorbent assay (ELISA) and a Western blot assay are useful in the immunodiagnosis of this infection, cross-reactivity remains a major problem. Recently, a recombinant<jats:named-content content-type=\"genus-species\">B. procyonis<\/jats:named-content>antigen, BpRAG1, was reported for use in the development of improved serological assays for the diagnosis of<jats:named-content content-type=\"genus-species\">Baylisascaris<\/jats:named-content>larva migrans. In this study, we tested a total of 384 human patient serum samples in a BpRAG1 ELISA, including samples from 20 patients with clinical<jats:named-content content-type=\"genus-species\">Baylisascaris<\/jats:named-content>larva migrans, 137 patients with other parasitic infections (8 helminth and 4 protozoan), and 227 individuals with unknown\/suspected parasitic infections. A sensitivity of 85% and a specificity of 86.9% were observed with the BpRAG1 ELISA, compared to only 39.4% specificity with the BPES ELISA. In addition, the BpRAG1 ELISA had a low degree of cross-reactivity with antibodies to<jats:named-content content-type=\"genus-species\">Toxocara<\/jats:named-content>infection (25%), while the BPES antigen showed 90.6% cross-reactivity. Based on these results, the BpRAG1 antigen has a high degree of sensitivity and specificity and should be very useful and reliable in the diagnosis and seroepidemiology of<jats:named-content content-type=\"genus-species\">Baylisascaris<\/jats:named-content>larva migrans by ELISA.<\/jats:p>","DOI":"10.1128\/cvi.00083-11","type":"journal-article","created":{"date-parts":[[2011,8,11]],"date-time":"2011-08-11T03:10:28Z","timestamp":1313032228000},"page":"1650-1655","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant Antigen-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Baylisascaris procyonis Larva Migrans"],"prefix":"10.1128","volume":"18","author":[{"given":"Sriveny","family":"Dangoudoubiyam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Vemulapalli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momar","family":"Ndao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin R.","family":"Kazacos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2011,8,10]]},"reference":[{"key":"key20180919073534_B1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2307\/3277335","article-title":"The nature of visceral larva migrans","volume":"55","author":"Beaver","year":"1969","journal-title":"J. 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