{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:18:00Z","timestamp":1759637880625},"reference-count":25,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A cDNA expression library of<jats:italic>Babesia gibsoni<\/jats:italic>was screened with the serum collected from a dog experimentally infected with<jats:italic>B. gibsoni<\/jats:italic>. A novel antigen sharing homology with secreted antigen 1 of<jats:italic>B. gibsoni<\/jats:italic>was isolated. The genomic analysis indicated that the BgSA3 gene exists as multicopies in the genome of<jats:italic>B. gibsoni<\/jats:italic>. The putative peptide encoded by the BgSA3 gene showed some characteristics of secreted proteins. The serum raised in mice immunized with the recombinant BgSA3 expressed in<jats:italic>Escherichia coli<\/jats:italic>could recognize a native parasite protein with a molecular mass of 70 kDa. Moreover, a sandwich enzyme-linked immunosorbent assay with anti-BgSA3 antibodies could detect the circulating BgSA3 in the blood plasma of dogs experimentally infected with<jats:italic>B. gibsoni<\/jats:italic>. The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens.<\/jats:p>","DOI":"10.1128\/cvi.00087-09","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T00:55:42Z","timestamp":1240448142000},"page":"944-948","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Identification of Secreted Antigen 3 from<i>Babesia gibsoni<\/i>"],"prefix":"10.1128","volume":"16","author":[{"given":"Honglin","family":"Jia","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Alaa","family":"Terkawi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Oluga","family":"Aboge","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youn-Kyoung","family":"Goo","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqing","family":"Ma","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinlin","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshifumi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Igarashi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kozo","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuenan","family":"Xuan","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"},{"name":"Qinghai Academy of Animal and Veterinary Science, Weier Road, Xining, Qinghai 810003, China"},{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Ziyue Road, Minhang District, Shanghai 200241, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"3","volume":"12","year":"2004","unstructured":"Alifrangis, M., F. T. Ihristensen, C. S. Jorgensen, A. M. Ronn, J. E. Weng, M. Chen, I. C. Bygjerg, W. Sirawaraporn, Y. Palarasah, and C. Koch. 2004. Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum. Malar. J.12:3-16.","journal-title":"Malar. J."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.actatropica.2005.03.005","volume":"95","year":"2005","unstructured":"Benito, A., and D. Carmena. 2005. Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs. Acta Trop.95:9-15.","journal-title":"Acta Trop."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"124s","DOI":"10.1007\/BF02632950","volume":"29","year":"1997","unstructured":"Beniwal, R. P., A. K. Nichani, N. K. Rakha, R. D. Sharma, and S. Sarup. 1997. An immunization trial with in vitro produced Babesia bigemina exoantigens. Trop. Anim. Health Prod.29:124s-126s.","journal-title":"Trop. Anim. Health Prod."},{"key":"e_1_3_2_5_2","first-page":"855","volume":"33","year":"2003","unstructured":"Boozer, A. L., and D. K. Macintire. 2003. Canine babesiosis. Vet. Clin. North Am. Small Anim. Pract.33:855-904.","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"e_1_3_2_6_2","first-page":"165","volume":"2","year":"2004","unstructured":"Bork, S., M. Okamura, S. Boonchit, H. Hirata, N. Yokoyama, and I. Igarashi. 2004. Identification of Babesia bovisl-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis. Mol. Biochem. Parasitol.2:165-172.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1136\/vr.142.7.168","volume":"142","year":"1998","unstructured":"Casapulla, R., L. Baldi, V. Avallone, R. Sannino, L. Pazzanese, and V. Mizzoni. 1998. Canine piroplasmosis due to Babesia gibsoni: clinical and morphological aspects. Vet. Rec.142:168-169.","journal-title":"Vet. Rec."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1179\/000349804225003181","volume":"98","year":"2004","unstructured":"De Arruda, M. E., K. M. Collins, L. P. Hochberg, P. R. Ryan, R. A. Wirtz, and J. R. Ryan. 2004. Quantitative determination of sporozoites and circumsporozoite antigen in mosquitoes infected with Plasmodium falciparum or P. vivax. Ann. Trop. Med. Parasitol.98:121-127.","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"e_1_3_2_9_2","first-page":"e204","volume":"8","year":"2005","unstructured":"Dondorp, A. M., V. Desakorn, W. Pongtavornpinyo, D. Sahassananda, K. Silamut, K. Chotivanich, P. N. Newton, P. Pitisuttithum, A. M. Smithyman, N. J. White, and N. P. Day. 2005. Estimation of the total parasite biomass in acute falciparum malaria from plasma PfHRP2. PLoS Med.8:e204.","journal-title":"PLoS Med."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2603-2609.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2947-2955.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.molbiopara.2004.02.006","volume":"136","year":"2004","unstructured":"Gaffar, F. R., A. P. Yatsuda, F. F. Franssen, and E. de Vries. 2004. A Babesia bovis merozoite protein with a domain architecture highly similar to the thrombospondin-related anonymous protein (TRAP) present in Plasmodium sporozoites. Mol. Biochem. Parasitol.136:25-34.","journal-title":"Mol. Biochem. Parasitol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.vetpar.2004.03.012","volume":"121","year":"2004","unstructured":"Inokuma, H., Y. Yoshizaki, K. Matsumoto, M. Okuda, T. Onishi, K. Nakagome, R. Kosugi, and M. Hirakawa. 2004. Molecular survey of Babesia infection in dogs in Okinawa. Jpn. Vet. Parasitol.121:341-346.","journal-title":"Jpn. Vet. Parasitol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"843","DOI":"10.4269\/ajtmh.2006.75.843","volume":"75","year":"2006","unstructured":"Jia, H., J. Zhou, H. Ikadai, A. Matsuu, H. Suzuki, I. Igarashi, K. Fujisaki, and X. Xuan. 2006. Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis. Am. J. Trop. Med. Hyg.75:843-850.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1007\/s00436-006-0392-8","volume":"100","year":"2007","unstructured":"Jia, H., M. Liao, E. Lee, Y. Nishikawa, H. Inokuma, H. Ikadai, A. Matsuu, I. Igarashi, and X. Xuan. 2007. Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs. Parasitol. Res.100:1381-1384.","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1007\/s004360050338","volume":"83","year":"1997","unstructured":"Johnson, W. C., L. E. Perryman, and W. L. Goff. 1997. Babesia bovis: identification of immunodominant merozoite surface proteins in soluble culture-derived exoantigen. Parasitol. Res.83:776-780.","journal-title":"Parasitol. Res."},{"key":"e_1_3_2_17_2","first-page":"745","volume":"4","year":"1992","unstructured":"Khusmith, S., P. Intapan, S. Tharavanij, S. Tuntrakul, K. A. Indravijit, and D. Bunnag. 1992. Two-site sandwich ELISA for detection of Plasmodium vivax blood stage antigens using monoclonal and polyclonal antibodies. Southeast Asian J. Trop. Med. Public Health4:745-751.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_18_2","first-page":"61","volume":"66","year":"1995","unstructured":"Lewis, B. D., B. L. Penzhorn, and L. M. Lopez Rebollar. 1995. Immune responses to South African Babesia canis and the development of a preliminary vaccine. J. S. Afr. Vet. Assoc.66:61-65.","journal-title":"J. S. Afr. Vet. Assoc."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1292\/jvms.67.467","volume":"67","year":"2005","unstructured":"Miyama, T., Y. Sakata, Y. Shimada, S. Ogino, M. Watanabe, K. Itamoto, M. Okuda, A. Verdida, X. Xuan, H. Nagasawa, and H. Inokuma. 2005. Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan. J. Vet. Med. Sci.67:467-471.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.1658-1664.2002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0304-4017(94)90114-7","volume":"52","year":"1994","unstructured":"Schettes, T. P., J. A. Kleuskens, N. C. Scholte, J. W. Pasman, and H. J. Bos. 1994. Vaccination of dogs against Babesia canis infection using antigens from culture supernatants with emphasis on clinical babesiosis. Vet. Parasitol.52:219-233.","journal-title":"Vet. Parasitol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.2.734-741.1993"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1292\/jvms.66.1517","volume":"66","year":"2004","unstructured":"Verdida, R. A., O. A. Hara, X. Xuan, S. Fukumoto, I. Igarashi, S. Zhang, J. Dong, H. Inokuma, H. Kabeya, Y. Sato, T. Moritomo, S. Maruyama, F. Claveria, and H. Nagasawa. 2004. Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50. J. Vet. Med. Sci.66:1517-1521.","journal-title":"J. Vet. Med. Sci."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0168-1702(96)01374-3","volume":"46","year":"1996","unstructured":"Xuan, X., K. Maeda, T. Mikami, and H. Otsuka. 1996. Characterization of canine herpesvirus glycoprotein C expressed in insect cells. Virus Res.46:57-64.","journal-title":"Virus Res."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.2460\/ajvr.1993.54.10.1579","volume":"54","year":"1993","unstructured":"Yamane, I., J. W. Thomford, I. A. Gardner, J. P. Dubey, M. Levy, and P. A. Conrad. 1993. Evaluation of the indirect fluorescent antibody test for diagnosis of Babesia gibsoni infections in dogs. Am. J. Vet. Res.54:1579-1584.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.vetpar.2006.01.037","volume":"138","year":"2006","unstructured":"Yokoyama, N., M. Okamura, and I. Igarashi. 2006. Erythrocyte invasion by Babesia parasites: current advances in the elucidation of the molecular interactions between the protozoan ligands and host receptors in the invasion stage. Vet. Parasitol.138:22-32.","journal-title":"Vet. Parasitol."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00087-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00087-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T20:57:11Z","timestamp":1710277031000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00087-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1128\/CVI.00087-09"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00087-09","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]},"assertion":[{"value":"2009-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}