{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T22:34:51Z","timestamp":1789425291723,"version":"build-2803163510"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>New or improved vaccines against dengue virus types 1 to 4 (DENV1 to DENV4) and Japanese encephalitis virus (JEV), the causative agents of dengue fever and Japanese encephalitis (JE), respectively, are urgently required. The use of noninfectious subviral extracellular particles (EPs) is an inexpensive and safe strategy for the production of protein-based flavivirus vaccines. Although coexpression of premembrane (prM) and envelope (E) proteins has been demonstrated to produce EPs in mammalian cells, low yields have hindered their commercial application. Therefore, we used an insect cell expression system with<jats:italic>Spodoptera frugiperda<\/jats:italic>-derived Sf9 cells to investigate high-level production of DENV2 and JEV EPs. Sf9 cells transfected with the<jats:italic>prM<\/jats:italic>and<jats:italic>E<\/jats:italic>genes of DENV2 or JEV secreted corresponding viral antigens in a particulate form that were biochemically and biophysically equivalent to the authentic antigens obtained from infected C6\/36 mosquito cells. Additionally, equivalent neutralizing antibody titers were induced in mice immunized either with EPs produced by transfected Sf9 cells or with EPs produced by transfected mammalian cells, in the context of coimmunization with a DNA vaccine that expresses EPs. Furthermore, the results of an enzyme-linked immunosorbent assay (ELISA) using an EP antigen derived from Sf9 cells correlated significantly with the results obtained by a neutralization test and an ELISA using an EP antigen derived from mammalian cells. Finally, Sf9 cells could produce 10- to 100-fold larger amounts of E antigen than mammalian cells. These results indicate the potential of Sf9 cells for high-level production of flavivirus protein vaccines and diagnostic antigens.<\/jats:p>","DOI":"10.1128\/cvi.00087-10","type":"journal-article","created":{"date-parts":[[2010,7,29]],"date-time":"2010-07-29T06:25:47Z","timestamp":1280384747000},"page":"1560-1566","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Evaluation of Extracellular Subviral Particles of Dengue Virus Type 2 and Japanese Encephalitis Virus Produced by<i>Spodoptera frugiperda<\/i>Cells for Use as Vaccine and Diagnostic Antigens"],"prefix":"10.1128","volume":"17","author":[{"given":"Miwa","family":"Kuwahara","sequence":"first","affiliation":[{"name":"Department of International Health, Kobe University Graduate School of Health Sciences"},{"name":"Division of Vaccinology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Konishi","sequence":"additional","affiliation":[{"name":"Department of International Health, Kobe University Graduate School of Health Sciences"},{"name":"Division of Vaccinology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/A:1026488408951","volume":"16","year":"1999","unstructured":"Altmann, F., E. Staudacher, I. B. Wilson, and L. M\u00e4rz. 1999. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj. J.16:109-123.","journal-title":"Glycoconj. J."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1016\/j.vaccine.2008.07.006","volume":"26","year":"2008","unstructured":"Babu, J. P., P. Pattnaik, N. Gupta, A. Shrivastava, M. Khan, and P. V. Lakshmana Rao. 2008. Immunogenicity of a recombinant envelope domain III protein of dengue virus type-4 with various adjuvants in mice. Vaccine26:4655-4663.","journal-title":"Vaccine"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1586\/14787210.4.2.313","volume":"4","year":"2006","unstructured":"Bharati, K., and S. Vrati. 2006. Japanese encephalitis: development of new candidate vaccines. Expert Rev. Anti Infect. Ther.4:313-324.","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.6.1150-1155.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4040-4047.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0065-3527(06)68004-4","volume":"68","year":"2006","unstructured":"Douris, V., L. Swevers, V. Labropoulou, E. Andronopoulou, Z. Georgoussi, and K. Iatrou. 2006. Stably transformed insect cell lines: tools for expression of secreted and membrane-anchored proteins and high-throughput screening platforms for drug and insecticide discovery. Adv. Virus Res.68:113-156.","journal-title":"Adv. Virus Res."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"353","DOI":"10.4269\/ajtmh.2008.79.353","volume":"79","year":"2008","unstructured":"Etemad, B., G. Batra, R. Raut, S. Dahiya, S. Khanam, S. Swaminathan, and N. Khanna. 2008. An envelope domain III-based chimeric antigen produced in Pichia pastoris elicits neutralizing antibodies against all four dengue virus serotypes. Am. J. Trop. Med. Hyg.79:353-363.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.vetimm.2006.05.012","volume":"113","year":"2006","unstructured":"Ge, F. F., Y. F. Qiu, X. Y. Gao, Y. W. Yang, and P. Y. Chen. 2006. Fusion expression of major antigenic segment of JEV E protein-hsp70 and the identification of domain acting as adjuvant in hsp70. Vet. Immunol. Immunopathol.113:288-296.","journal-title":"Vet. Immunol. Immunopathol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"548","DOI":"10.4269\/ajtmh.1982.31.548","volume":"31","year":"1982","unstructured":"Gentry, M. K., E. A. Henchal, J. M. McCown, W. E. Brandt, and J. M. Dalrymple. 1982. Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. Am. J. Trop. Med. Hyg.31:548-555.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_11_2","first-page":"1153","year":"2007","unstructured":"Gubler, D. J., G. Kuno, and L. Markoff. 2007. Flaviviruses, p 1153-1252. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1016\/S0140-6736(07)61687-0","volume":"370","year":"2007","unstructured":"Halstead, S. B. 2007. Dengue. Lancet370:1644-1652.","journal-title":"Lancet"},{"key":"e_1_3_2_13_2","first-page":"1155","year":"2008","unstructured":"Halstead, S. B., and D. W. Vaughn. 2008. Dengue vaccines, p. 1155-1161. In S. A. Plotkin, W. A. Orenstein, and P. A. Offit (ed.), Vaccines, 5th ed. Saunders Elsevier, Maryland Heights, MO.","journal-title":"Vaccines"},{"key":"e_1_3_2_14_2","first-page":"311","year":"2008","unstructured":"Halstead, S. B., and J. Jacobsen. 2008. Japanese encephalitis vaccines, p 311-352. In S. A. Plotkin, W. A. Orenstein, and P. A. Offit (ed.), Vaccines, 5th ed. Saunders Elsevier, Maryland Heights, MO.","journal-title":"Vaccines"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4269\/ajtmh.1985.34.162","volume":"34","year":"1985","unstructured":"Henchal, E. A., J. M. McCown, D. S. Burke, M. C. Seguin, and W. E. Brandt. 1985. Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. Am. J. Trop. Med. Hyg.34:162-169.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0166-0934(01)00346-9","volume":"97","year":"2001","unstructured":"Hunt, A. R., C. B. Cropp, and G. J. Chang. 2001. A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen. J. Virol. Methods97:133-149.","journal-title":"J. Virol. Methods"},{"key":"e_1_3_2_17_2","first-page":"139","volume":"267","year":"2002","unstructured":"Igarashi, A. 2002. Control of Japanese encephalitis in Japan: immunization of humans and animals, and vector control. Curr. Top. Microbiol. Immunol.267:139-152.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00253-003-1223-9","volume":"62","year":"2003","unstructured":"Ikonomou, L., Y. J. Schneider, and S. N. Agathos. 2003. Insect cell culture for industrial production of recombinant proteins. Appl. Microbiol. Biotechnol.62:1-20.","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1089\/vim.2005.18.205","volume":"18","year":"2005","unstructured":"Imoto, J., and E. Konishi. 2005. Needle-free jet injection of a mixture of Japanese encephalitis DNA and protein vaccine in a murine model. Viral Immunol.18:205-212.","journal-title":"Viral Immunol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.vaccine.2006.09.059","volume":"25","year":"2007","unstructured":"Imoto, J., and E. Konishi. 2007. Dengue tetravalent DNA vaccine increases its immunogenicity in mice when mixed with a dengue type 2 subunit vaccine or an inactivated Japanese encephalitis vaccine. Vaccine25:1076-1084.","journal-title":"Vaccine"},{"key":"e_1_3_2_21_2","first-page":"2133","volume":"63","year":"2005","unstructured":"Ishikawa, T. 2005. Development of new Japanese encephalitis vaccine. Nippon Rinsho63:2133-2137. (In Japanese.)","journal-title":"Nippon Rinsho"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1348-0421.2006.tb03788.x","volume":"50","year":"2006","unstructured":"Ishikawa, T., and E. Konishi. 2006. Mosquito cells infected with Japanese encephalitis virus release slowly-sedimenting hemagglutinin particles in association with intracellular formation of smooth membrane structures. Microbiol. Immunol.50:211-223.","journal-title":"Microbiol. Immunol."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/j.micinf.2007.05.013","volume":"9","year":"2007","unstructured":"Ishikawa, T., T. Takasaki, I. Kurane, S. Nukuzuma, T. Kondo, and E. Konishi. 2007. Co-immunization with West Nile DNA and inactivated vaccines provides synergistic increases in their immunogenicities in mice. Microbes Infect.9:1089-1095.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"2549","DOI":"10.1016\/S0264-410X(00)00032-3","volume":"18","year":"2000","unstructured":"Kelly, E. P., J. J. Greene, A. D. King, and B. L. Innis. 2000. Purified dengue 2 virus envelope glycoprotein aggregates produced by baculovirus are immunogenic in mice. Vaccine18:2549-2559.","journal-title":"Vaccine"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.16.8745-8755.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1517\/14712590903042282","volume":"9","year":"2009","unstructured":"Kollaritsch, H., M. Paulke-Korinek, and K. Dubischar-Kastner. 2009. IC51 Japanese encephalitis vaccine. Expert Opin. Biol. Ther.9:921-931.","journal-title":"Expert Opin. Biol. Ther."},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1016\/S0264-410X(01)00446-7","volume":"20","year":"2002","unstructured":"Konishi, E., and A. Fujii. 2002. Dengue type 2 virus subviral extracellular particles produced by a stably transfected mammalian cell line and their evaluation for a subunit vaccine. Vaccine20:1058-1067.","journal-title":"Vaccine"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.5.2204-2212.2001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"3713","DOI":"10.1016\/S0264-410X(03)00376-1","volume":"21","year":"2003","unstructured":"Konishi, E., A. Terazawa, and A. Fujii. 2003. Evidence for antigen production in muscles by dengue and Japanese encephalitis DNA vaccines and a relation to their immunogenicity in mice. Vaccine21:3713-3720.","journal-title":"Vaccine"},{"key":"e_1_3_2_30_2","first-page":"1","volume":"2","year":"2000","unstructured":"Konishi, E., I. Kurane, and P. W. Mason. 2000. Immune response to traditional and genetically engineered Japanese encephalitis vaccines. Recent Res. Dev. Virol.2:1-21.","journal-title":"Recent Res. Dev. Virol."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"410","DOI":"10.7883\/yoken.JJID.2008.410","volume":"61","year":"2008","unstructured":"Konishi, E., K. Yagawa, and A. Yamanaka. 2008. Vero cells infected with vaccinia viruses expressing Japanese encephalitis virus envelope protein induce polykaryocyte formation under neutral conditions. Jpn. J. Infect. Dis.61:410-411.","journal-title":"Jpn. J. Infect. Dis."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5087-5093.2004"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1002\/(SICI)1096-9071(199601)48:1<76::AID-JMV12>3.0.CO;2-4","volume":"48","year":"1996","unstructured":"Konishi, E., P. W. Mason, and R. E. Shope. 1996. Enzyme-linked immunosorbent assay using recombinant antigens for serodiagnosis of Japanese encephalitis. J. Med. Virol.48:76-79.","journal-title":"J. Med. Virol."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/0042-6822(92)90526-U","volume":"188","year":"1992","unstructured":"Konishi, E., S. Pincus, E. Paoletti, R. E. Shope, T. Burrage, and P. W. Mason. 1992. Mice immunized with a subviral particle containing the Japanese encephalitis virus prM\/M and E proteins are protected from lethal JEV infection. Virology188:714-720.","journal-title":"Virology"},{"key":"e_1_3_2_35_2","first-page":"91","volume":"267","year":"2002","unstructured":"Kurane, I. 2002. Immune responses to Japanese encephalitis virus. Curr. Top. Microbiol. Immunol.267:91-103.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"4519","DOI":"10.1016\/S0264-410X(03)00506-1","volume":"21","year":"2003","unstructured":"Kuzuhara, S., H. Nakamura, K. Hayashida, J. Obata, M. Abe, K. Sonoda, K. Nishiyama, K. Sugawara, K. Takeda, T. Honda, H. Matsui, T. Shigaki, Y. Kino, H. Mizokami, M. Tanaka, K. Mizuno, and K. Ueda. 2003. Non-clinical and phase I clinical trials of a Vero cell-derived inactivated Japanese encephalitis vaccine. Vaccine21:4519-4526.","journal-title":"Vaccine"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"3915","DOI":"10.1016\/j.vaccine.2005.03.006","volume":"23","year":"2005","unstructured":"Ledizet, M., K. Kar, H. G. Foellmer, T. Wang, S. L. Bushmich, J. F. Anderson, E. Fikrig, and R. A. Koski. 2005. A recombinant envelope protein vaccine against West Nile virus. Vaccine23:3915-3924.","journal-title":"Vaccine"},{"key":"e_1_3_2_38_2","first-page":"1101","year":"2007","unstructured":"Lindenbach, B. D., H.-J. Thiel, and C. M. Rice. 2007. Flaviviridae: the viruses and their replication, p. 1101-1152. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s11262-009-0418-2","volume":"40","year":"2010","unstructured":"Liu, W., H. Jiang, J. Zhou, X. Yang, Y. Tang, D. Fang, and L. Jiang. 2010. Recombinant dengue virus-like particles from Pichia pastoris: efficient production and immunological properties. Virus Genes40:53-59.","journal-title":"Virus Genes"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1080\/13550280591002487","volume":"11","year":"2005","unstructured":"Mackenzie, J. S. 2005. Emerging zoonotic encephalitis viruses: lessons from Southeast Asia and Oceania. J. Neurovirol.11:434-440.","journal-title":"J. Neurovirol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1099\/0022-1317-70-8-2037","volume":"70","year":"1989","unstructured":"Mason, P. W., J. M. Dalrymple, M. K. Gentry, J. M. McCown, C. H. Hoke, D. D. Burke, M. J. Fournier, and T. L. Mason. 1989. Molecular characterization of a neutralizing domain of the Japanese encephalitis virus structural glycoprotein. J. Gen. Virol.70:2037-2049.","journal-title":"J. Gen. Virol."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/0042-6822(91)90034-9","volume":"180","year":"1991","unstructured":"Mason, P. W., S. Pincus, M. J. Fournier, T. L. Mason, R. E. Shope, and E. Paoletti. 1991. Japanese encephalitis virus-vaccinia recombinants produce particulate forms of the structural membrane proteins and induce high levels of protection against lethal JEV infection. Virology180:294-305.","journal-title":"Virology"},{"key":"e_1_3_2_43_2","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1086\/517613","volume":"195","year":"2007","unstructured":"McDonald, W. F., J. W. Huleatt, H. G. Foellmer, D. Hewitt, J. Tang, P. Desai, A. Price, A. Jacobs, V. N. Takahashi, Y. Huang, V. Nakaar, L. Alexopoulou, E. Fikrig, and T. J. Powell. 2007. A West Nile virus recombinant protein vaccine that coactivates innate and adaptive immunity. J. Infect. Dis.195:1607-1617.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1016\/j.vaccine.2003.12.008","volume":"22","year":"2004","unstructured":"Mutoh, E., T. Ishikawa, A. Takamizawa, T. Kurata, T. Sata, and A. Kojima. 2004. Japanese encephalitis subunit vaccine composed of virus-like envelope antigen particles purified from serum-free medium of a high-producer J12#26 cell clone. Vaccine22:2599-2608.","journal-title":"Vaccine"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1708-8305.2007.00134.x","volume":"14","year":"2007","unstructured":"Oya, A., and I. Kurane. 2007. Japanese encephalitis for a reference to international travelers. J. Travel Med.14:259-268.","journal-title":"J. Travel Med."},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1016\/S0140-6736(07)60946-5","volume":"369","year":"2007","unstructured":"Paavonen, J., D. Jenkins, F. X. Bosch, P. Naud, J. Salmer\u00f3n, C. M. Wheeler, S. N. Chow, D. L. Apter, H. C. Kitchener, X. Castellsague, N. S. de Carvalho, S. R. Skinner, D. M. Harper, J. A. Hedrick, U. Jaisamrarn, G. A. Limson, M. Dionne, W. Quint, B. Spiessens, P. Peeters, F. Struyf, S. L. Wieting, M. O. Lehtinen, and G. Dubin; HPV PATRICIA study group. 2007. Efficacy of a prophylactic adjuvanted bivalent L1 virus-like-particle vaccine against infection with human papillomavirus types 16 and 18 in young women: an interim analysis of a phase III double-blind, randomised controlled trial. Lancet369:2161-2170.","journal-title":"Lancet"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"e12","DOI":"10.1017\/S1462399408000665","volume":"10","year":"2008","unstructured":"Pierson, T. C., and M. S. Diamond. 2008. Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection. Expert Rev. Mol. Med.10:e12.","journal-title":"Expert Rev. Mol. Med."},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0065-3527(03)61012-2","volume":"61","year":"2003","unstructured":"Putnak, R., K. Porter, and C. Schmaljohn. 2003. DNA vaccines for flaviviruses. Adv. Virus. Res.61:445-468.","journal-title":"Adv. Virus. Res."},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1086\/425933","volume":"190","year":"2004","unstructured":"Qiao, M., M. Ashok, K. A. Bernard, G. Palacios, Z. H. Zhou, W. I. Lipkin, and T. J. Liang. 2004. Induction of sterilizing immunity against West Nile Virus (WNV), by immunization with WNV-like particles produced in insect cells. J. Infect. Dis.190:2104-2108.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.jcv.2005.11.004","volume":"35","year":"2006","unstructured":"Ravi, V., A. Desai, M. Balaji, M. P. Apte, L. Lakshman, D. K. Subbakrishna, G. Sridharan, T. N. Dhole, and B. V. Ravikumar. 2006. Development and evaluation of a rapid IgM capture ELISA (JEV-Chex) for the diagnosis of Japanese encephalitis. J. Clin. Virol.35:429-434.","journal-title":"J. Clin. Virol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1263\/jbb.103.572","volume":"103","year":"2007","unstructured":"Shishido, T., M. Muraoka, H. Yamaji, A. Kondo, and H. Fukuda. 2007. Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles. J. Biosci. Bioeng.103:572-574.","journal-title":"J. Biosci. Bioeng."},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"1946","DOI":"10.1016\/S0264-410X(97)00128-X","volume":"15","year":"1997","unstructured":"Staropoli, I., M. P. Frenkiel, F. M\u00e9gret, and V. Deubel. 1997. Affinity-purified dengue-2 virus envelope glycoprotein induces neutralizing antibodies and protective immunity in mice. Vaccine15:1946-1954.","journal-title":"Vaccine"},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.micinf.2009.07.009","volume":"11","year":"2009","unstructured":"Takahashi, H., N. Ohtaki, M. Maeda-Sato, M. Tanaka, K. Tanaka, H. Sawa, T. Ishikawa, A. Takamizawa, T. Takasaki, H. Hasegawa, T. Sata, W. W. Hall, T. Kurata, and A. Kojima. 2009. Effects of the number of amino acid residues in the signal segment upstream or downstream of the NS2B-3 cleavage site on production and secretion of prM\/M-E virus-like particles of West Nile virus. Microbes Infect.11:1019-1028.","journal-title":"Microbes Infect."},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0022-1759(98)00077-5","volume":"216","year":"1998","unstructured":"Verma, R., E. Boleti, and A. J. George. 1998. Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. J. Immunol. Methods216:165-181.","journal-title":"J. Immunol. Methods"},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"6905","DOI":"10.1016\/j.vaccine.2009.09.006","volume":"27","year":"2009","unstructured":"Verma, S. K., S. Kumar, N. Gupta, S. Vedi, S. M. Bhattacharya, and P. V. Lakshmana Rao. 2009. Bacterially expressed recombinant envelope protein domain III of Japanese encephalitis virus (rJEV-DIII) elicits Th1 type of immune response in BALB\/c mice. Vaccine27:6905-6909.","journal-title":"Vaccine"},{"key":"e_1_3_2_56_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0065-3527(08)00402-8","volume":"72","year":"2008","unstructured":"Widman, D. G., I. Frolov, and P. W. Mason. 2008. Third-generation flavivirus vaccines based on single-cycle, encapsidation-defective viruses. Adv. Virus Res.72:77-126.","journal-title":"Adv. Virus Res."},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4142\/jvs.2005.6.2.125","volume":"6","year":"2005","unstructured":"Yang, D. K., C. H. Kweon, B. H. Kim, S. I. Lim, J. H. Kwon, S. H. Kim, J. Y. Song, and H. R. Han. 2005. Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice. J. Vet. Sci.6:125-133.","journal-title":"J. Vet. Sci."},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jviromet.2007.02.012","volume":"143","year":"2007","unstructured":"Zhang, Z. S., Y. S. Yan, Y. W. Weng, H. L. Huang, S. Q. Li, S. He, and J. M. Zhang. 2007. High-level expression of recombinant dengue virus type 2 envelope domain III protein and induction of neutralizing antibodies in BALB\/c mice. J. Virol. Methods143:125-131.","journal-title":"J. Virol. Methods"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00087-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00087-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T12:44:26Z","timestamp":1685709866000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00087-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":57,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1128\/CVI.00087-10"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00087-10","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10]]},"assertion":[{"value":"2010-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}