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The influence of receptor type on susceptibility to anthrax toxin components was examined using Chinese hamster ovary (CHO) cells expressing the human form of one of two PA receptors: TEM8 or CMG2. Unexpectedly, PA alone, previously believed to only mediate entry of lethal factor or edema factor, was found to be toxic to CHO-TEM8 cells; cells treated with PA alone displayed reduced cell growth and decreased metabolic activity. PA-treated cells swelled and became permeable to membrane-excluded dye, suggesting that PA formed cell surface pores on CHO-TEM8 cells. While CHO-CMG2 cells were not killed by wild-type PA, they were susceptible to the PA variant, F427A. Receptor expression also conferred differences in susceptibility to edema factor.<\/jats:p>","DOI":"10.1128\/cvi.00103-08","type":"journal-article","created":{"date-parts":[[2008,7,3]],"date-time":"2008-07-03T01:06:35Z","timestamp":1215047195000},"page":"1330-1336","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Toxicity of Anthrax Toxin Is Influenced by Receptor Expression"],"prefix":"10.1128","volume":"15","author":[{"given":"Sarah C.","family":"Taft","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison A.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0524"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1083\/jcb.200211018","volume":"160","year":"2003","unstructured":"Abrami, L., S. 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