{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:06:10Z","timestamp":1789985170624,"version":"4.0.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Melioidosis is a severe infectious disease caused by<jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>. It is highly resistant to antibiotic treatment, and there is currently no licensed vaccine.<jats:named-content content-type=\"genus-species\">Burkholderia thailandensis<\/jats:named-content>is a close relative of<jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>but is essentially avirulent in mammals. In this report, we detail the protective efficacy of immunization with live<jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>E555, a strain which has been shown to express an antigenic capsule similar to that of<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>. Immunization with E555 induced significant protection against a lethal intraperitoneal<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>challenge in a mouse model of infection, with no mice succumbing to infection over the course of the study, even with challenges of up to 6,000 median lethal doses. By comparison, mice immunized with<jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>not expressing a<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>-like capsule had significantly decreased levels of protection. E555-immunized mice had significantly higher levels of IgG than mice immunized with noncapsulated<jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>, and these antibody responses were primarily directed against the capsule.<\/jats:p>","DOI":"10.1128\/cvi.00113-13","type":"journal-article","created":{"date-parts":[[2013,5,16]],"date-time":"2013-05-16T04:28:29Z","timestamp":1368678509000},"page":"1041-1047","source":"Crossref","is-referenced-by-count":37,"title":["Protection against Experimental Melioidosis following Immunization with Live Burkholderia thailandensis Expressing a<i>manno<\/i>-Heptose Capsule"],"prefix":"10.1128","volume":"20","author":[{"given":"Andrew E.","family":"Scott","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Laws","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo V.","family":"D'Elia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret G. M.","family":"Stokes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tannistha","family":"Nandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Diane","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joann L.","family":"Prior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2013,5,15]]},"reference":[{"key":"key20180919073657_B1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005","journal-title":"Clin. Microbiol. 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