{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:10:47Z","timestamp":1791407447634,"version":"4.3.3"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The environmental Gram-negative encapsulated bacillus<jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>is the causative agent of melioidosis, a disease associated with high morbidity and mortality rates in areas of Southeast Asia and northern Australia in which the disease is endemic.<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>is also classified as a tier I select agent due to the high level of lethality of the bacterium and its innate resistance to antibiotics, as well as the lack of an effective vaccine. Gram-negative bacteria, including<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>, secrete outer membrane vesicles (OMVs) which are enriched with multiple protein, lipid, and polysaccharide antigens. Previously, we demonstrated that immunization with multivalent<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>-derived OMVs protects highly susceptible BALB\/c mice against an otherwise lethal aerosol challenge. In this work, we evaluated the protective efficacy of OMV immunization against intraperitoneal challenge with a heterologous strain because systemic infection with phenotypically diverse environmental<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>strains poses another hazard and a challenge to vaccine development. We demonstrated that<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>OMVs derived from strain 1026b afforded significant protection against septicemic infection with<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>strain K96243. OMV immunization induced robust OMV-, lipopolysaccharide-, and capsular polysaccharide-specific serum IgG (IgG1, IgG2a, and IgG3) and IgM antibody responses. OMV-immune serum promoted bacterial killing<jats:italic>in vitro<\/jats:italic>, and passive transfer of<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>OMV immune sera protected naive mice against a subsequent challenge. These results indicate that OMV immunization provides antibody-mediated protection against acute, rapidly lethal sepsis in mice.<jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>-derived OMVs may represent an efficacious multivalent vaccine strategy against melioidosis.<\/jats:p>","DOI":"10.1128\/cvi.00119-14","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T03:45:45Z","timestamp":1395891945000},"page":"747-754","source":"Crossref","is-referenced-by-count":86,"title":["A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Protection against Lethal Sepsis"],"prefix":"10.1128","volume":"21","author":[{"given":"Wildaliz","family":"Nieves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara M.","family":"Judy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla A.","family":"Blumentritt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasi","family":"Russell-Lodrigue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad J.","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo G.","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Morici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2014,3,26]]},"reference":[{"key":"key20180919073732_B1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1056\/NEJMra1204699","article-title":"Melioidosis","volume":"367","author":"Wiersinga","year":"2012","journal-title":"N. 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