{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:47:00Z","timestamp":1788572820106,"version":"build-2803163510"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2007,7,1]],"date-time":"2007-07-01T00:00:00Z","timestamp":1183248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In the absence of erythema migrans, the basis for diagnosis of Lyme disease is the demonstration of an antibody response against<jats:italic>Borrelia burgdorferi<\/jats:italic>in an appropriate clinical setting. The C6 enzyme-linked immunosorbent assay, based on the IR6 region of VlsE, has become widely used in both the United States and Europe. We mapped the antigenic epitopes of IR6 to a shorter sequence that is equivalent in sensitivity and specificity to the full-length IR6 25-residue peptide. In addition, we observed significant differences in sensitivity between serum panels (60 to 100%), indicating that the selection of the serum panels can shape the apparent overall sensitivity of the assay. Contrary to prior reports, the assay sensitivity is greater when the IR6 peptide is derived from the sequence of the same infecting<jats:italic>Borrelia<\/jats:italic>genospecies. Using our North American panels and the two panels obtained from European Lyme disease patients, we determined that the IR6 assay that is based on a single genospecies of<jats:italic>Borrelia<\/jats:italic>spp. is not optimal for use as a universal diagnostic assay for Lyme disease.<\/jats:p>","DOI":"10.1128\/cvi.00122-07","type":"journal-article","created":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T01:19:27Z","timestamp":1180574367000},"page":"875-879","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Epitope Length, Genospecies Dependency, and Serum Panel Effect in the IR6 Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"14","author":[{"given":"Maria J. 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