{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T18:06:52Z","timestamp":1749578812746},"reference-count":19,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The performance of an immunofiltration assay (IMFA) that detects immunoglobulin M (IgM) antibodies to the Epstein-Barr virus (EBV) ZEBRA (BamHI Z EBV replication activator) protein was evaluated for the diagnosis of EBV infectious mononucleosis (IM) in immunocompetent patients. The test panel consisted of 47 sera displaying an EBV-specific antibody profile compatible with an acute primary EBV infection from patients with clinical and biological features of EBV IM, 20 sera from healthy individuals either with a past EBV infection or who were EBV seronegative, 20 sera displaying an equivocal EBV antibody pattern (viral capsid antigen IgG positive [VCA IgG<jats:sup>+<\/jats:sup>], VCA IgM<jats:sup>+<\/jats:sup>, and EBV nuclear antigen-1 IgG<jats:sup>+<\/jats:sup>), and 15 sera obtained from patients with a mononucleosis-like syndrome owing to cytomegalovirus, human herpesvirus 6, or parvovirus B19. Overall, the sensitivity and the specificity of the assay were found to be 92.5%, and 97.3%, respectively. The sensitivity of the assay for the diagnosis of heterophile antibody-negative EBV IM was 86.2%. The IMFA is rapid, easy to perform, and, thus, suitable for point-of-care testing, and it may be used as a first-line test for the diagnosis of acute EBV IM in immunocompetent patients.<\/jats:p>","DOI":"10.1128\/cvi.00123-09","type":"journal-article","created":{"date-parts":[[2009,4,30]],"date-time":"2009-04-30T01:25:41Z","timestamp":1241054741000},"page":"885-888","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of an Immunofiltration Assay That Detects Immunoglobulin M Antibodies against the ZEBRA Protein for the Diagnosis of Epstein-Barr Virus Infectious Mononucleosis in Immunocompetent Patients"],"prefix":"10.1128","volume":"16","author":[{"given":"Dayana","family":"Bravo","sequence":"first","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Mun\u0303oz-Cobo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Angeles","family":"Clari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Tormo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital Cli\u0301nico Universitario, Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/(SICI)1096-9071(199811)56:3<186::AID-JMV2>3.0.CO;2-3","volume":"56","year":"1998","unstructured":"Aalto, S. M., K. Linnavuori, H. Peltola, E. Vuori, B. Weissbrich, J. Shubert, L. Hedman, and K. Hedman. 1998. Immunoreactivation of Epstein-Barr virus due to cytomegalovirus primary infection. J. Med. Virol.56:186-191.","journal-title":"J. Med. Virol."},{"key":"e_1_3_1_3_2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1002\/jmv.20237","volume":"75","year":"2005","unstructured":"Bauer, C. C., S. W. Aberle, T. Popow-Kraupp, M. Kapitan, H. Hofmann, and E. Puchhammer-St\u00f6ckl. 2005. Serum Epstein-Barr virus DNA load in primary Epstein-Barr virus infection. J. Med. Virol.75:54-58.","journal-title":"J. Med. Virol."},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00082-08"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4152-4154;2001"},{"key":"e_1_3_1_6_2","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1073\/pnas.82.12.4085","volume":"82","year":"1985","unstructured":"Countryman, J., and G. Miller. 1985. Activation of expression of latent Epstein-Barr virus after gene transfer of a small subfragment of heterogeneous viral DNA. Proc. Natl. Acad. Sci. USA82:4085-4089.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.6.1669-1671.1993"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.5.840-841.2000"},{"key":"e_1_3_1_9_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0166-0934(94)00147-9","volume":"52","year":"1995","unstructured":"Gray, J. J. 1995. Avidity of EBV VCA-specific IgG antibodies: distinction between recent primary infection, past infection and reactivation. J. Virol. Methods52:95-104.","journal-title":"J. Virol. Methods"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3381-3387.2004"},{"key":"e_1_3_1_11_2","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1007\/BF01963758","volume":"8","year":"1989","unstructured":"Ijsselmuiden, O. E., G. Beelaert, L. M. Schouls, B. Tank, E. Stolz, and G. van der Groen. 1989. Line immunoassay and enzyme-linked line immunofiltration assay for simultaneous detection of antibody to two treponemal antigens. Eur. J. Clin. Microbiol. Infect. Dis.8:716-721.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_1_12_2","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/ijc.2910480503","volume":"48","year":"1991","unstructured":"Joab, I., J. C. Nicolas, G. Schwabb, G. de Th\u00e9, B. Clausse, M. Perricaudet, and Y. Zeng. 1991. Detection of anti-Epstein-Barr virus transactivator (ZEBRA) antibodies in sera from patients with nasopharyngeal carcinoma. Int. J. Cancer48:647-649.","journal-title":"Int. J. Cancer"},{"key":"e_1_3_1_13_2","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1002\/jmv.20715","volume":"78","year":"2006","unstructured":"Navalpotro, D., C. Gimeno, and D. Navarro. 2006. Concurrent detection of human herpesvirus type 6 and measles-specific IgMs during acute exanthematic human parvovirus B19 infection. J. Med. Virol.78:1449-1451.","journal-title":"J. Med. Virol."},{"key":"e_1_3_1_14_2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.jcv.2007.01.006","volume":"38","year":"2007","unstructured":"Nystad, T. W., and H. Myrmel. 2007. Prevalence of primary versus reactivated Epstein-Barr virus infection in patients with VCA IgG-, VCA IgM-, and EBNA-1-antibodies and suspected infectious mononucleosis. J. Clin. Virol.38:292-297.","journal-title":"J. Clin. Virol."},{"key":"e_1_3_1_15_2","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1542\/peds.75.6.1011","volume":"75","year":"1985","unstructured":"Sumaya, C. V., and Y. Ench. 1985. Epstein-Barr infectious mononucleosis in children. II. Heterophile antibody and viral-specific responses. Pediatrics75:1011-1019.","journal-title":"Pediatrics"},{"key":"e_1_3_1_16_2","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1099\/0022-1317-76-6-1393","volume":"76","year":"1995","unstructured":"Tedeschi, R., Y. T. Foong, H. M. Cheng, P. de Paoli, T. Lehtinen, T. Elfborg, and J. Dillner. 1995. The disease associations of the antibody response against the Epstein-Barr virus transactivator protein ZEBRA can be separated into different epitopes. J. Gen. Virol.76:1393-1400.","journal-title":"J. Gen. Virol."},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S1386-6532(99)00051-7","volume":"14","year":"1999","unstructured":"Thomas, H. I., E. Barret, L. M. Hesketh, A. Wynne, and P. Morgan-Capner. 1999. Simultaneous IgM reactivity by EIA against more than one virus in measles, parvovirus B19 and rubella infection. J. Clin. Virol.14:107-118.","journal-title":"J. Clin. Virol."},{"key":"e_1_3_1_18_2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.clinbiochem.2005.03.008","volume":"38","year":"2005","unstructured":"von Lode, P. 2005. Point-of-care immunotesting: approaching the analytical performance of central laboratory methods. Clin. Biochem.38:591-606.","journal-title":"Clin. Biochem."},{"key":"e_1_3_1_19_2","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1159\/000024950","volume":"41","year":"1998","unstructured":"Yamauchi, Y., Y. Tachiband, A. Maeda, H. Wakiguchi, M. Usui, T. Kurata, and T. Sairenji. 1998. Evaluation of antibodies to the Epstein-Barr immediate early gene product ZEBRA by a new enzyme-linked immunosorbent assay. Intervirology41:278-284.","journal-title":"Intervirology"},{"key":"e_1_3_1_20_2","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1097\/00006454-199911000-00007","volume":"18","year":"1999","unstructured":"Zufferey, J., P. Hohlfeld, J. Bille, C. L. Fawer, D. Blanc, J. M. Pinon, and B. Vaudaux. 1999. Value of the comparative enzyme-linked immunofiltration assay for early neonatal diagnosis of congenital Toxoplasma infection. Pediatr. Infect. Dis. J.18:971-975.","journal-title":"Pediatr. Infect. Dis. J."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00123-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00123-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T23:42:36Z","timestamp":1684971756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00123-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1128\/CVI.00123-09"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00123-09","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]},"assertion":[{"value":"2009-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-04-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}