{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:15:47Z","timestamp":1791033347699,"version":"4.1.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In France and Finland, farmer's lung disease (FLD), a hypersensitivity pneumonitis common in agricultural areas, is mainly caused by\n            <jats:italic>Eurotium<\/jats:italic>\n            species. The presence of antibodies in patients' serum is an important criterion for diagnosis. Our study aimed to improve the serological diagnosis of FLD by using common fungal particles that pollute the farm environment as antigens. Fungal particles of the\n            <jats:italic>Eurotium<\/jats:italic>\n            species were observed in handled hay. A strain of\n            <jats:italic>Eurotium amstelodami<\/jats:italic>\n            was grown\n            <jats:italic>in vitro<\/jats:italic>\n            using selected culture media; and antigen extracts from sexual (ascospores), asexual (conidia), and vegetative (hyphae) forms were made. Antigens were tested by enzyme-linked immunosorbent assay (ELISA), which was used to test for immunoglobulin G antibodies from the sera of 17 FLD patients, 40 healthy exposed farmers, and 20 nonexposed controls. The antigens were compared by receiver operating characteristic analysis, and a threshold was then established. The ascospores contained in asci enclosed within cleistothecia were present in 38% of the hay blades observed; conidial heads of aspergillus were less prevalent. The same protocol was followed to make the three antigen extracts. A comparison of the results for FLD patients and exposed controls showed the area under the curve to be 0.850 for the ascospore antigen, 0.731 for the conidia, and 0.690 for the hyphae. The cutoffs that we determined, with the standard deviation for measures being taken into account, showed 67% for sensitivity and 92% for specificity with the ascospore antigen. In conclusion, the serological diagnosis of FLD by ELISA was improved by the adjunction of ascospore antigen.\n          <\/jats:p>","DOI":"10.1128\/cvi.00129-09","type":"journal-article","created":{"date-parts":[[2009,11,12]],"date-time":"2009-11-12T03:17:06Z","timestamp":1257995826000},"page":"160-167","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of Three Antigenic Extracts of\n            <i>Eurotium amstelodami<\/i>\n            in Serological Diagnosis of Farmer's Lung Disease"],"prefix":"10.1128","volume":"17","author":[{"given":"Sandrine","family":"Roussel","sequence":"first","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"},{"name":"Department of Dermatology, University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Reboux","sequence":"additional","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"},{"name":"Department of Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Be\u0301ne\u0301dicte","family":"Rognon","sequence":"additional","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Monod","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Grenouillet","sequence":"additional","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"},{"name":"Department of Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfredo","family":"Quadroni","sequence":"additional","affiliation":[{"name":"Protein Analysis Facility, Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Fellrath","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, University Hospital, Neuchatel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John-David","family":"Aubert","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Charles","family":"Dalphin","sequence":"additional","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"},{"name":"Department of Respiratory Disease, University Hospital, Besancon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"UMR Chrono-Environnement 6249\/CNRS, University of Besancon, Besancon, France"},{"name":"Department of Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"272","volume":"32","year":"1978","unstructured":"Blyth, W. 1978. 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