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One immunoreactive phage clone contained a gene (<jats:italic>p26<\/jats:italic>) with significant nucleotide identity with orthologs in brucellae, bartonellae, and several plant-associated bacteria.<jats:italic>p26<\/jats:italic>gene sequences from four<jats:italic>B. henselae<\/jats:italic>strains, one<jats:italic>B. koehlerae<\/jats:italic>strain, and one<jats:italic>B. clarridgeiae<\/jats:italic>strain were cloned. Comparative nucleotide sequence analysis showed that<jats:italic>p26<\/jats:italic>is a potential marker for molecular diagnosis of infection, as well as for identification to species level and genotyping of<jats:italic>Bartonella<\/jats:italic>sp. isolates. Alignment of the predicted amino acid sequences illustrated conserved putative protein features including a hydrophobic transmembrane region, a peptide cleavage site, and four dominant antigenic sites. Expression of<jats:italic>p26<\/jats:italic>in<jats:italic>Escherichia coli<\/jats:italic>produced two proteins (26 and 27.5 kDa), both of which were reactive with feline anti-<jats:italic>B. henselae<\/jats:italic>antisera. Furthermore, murine hyperimmune serum raised against either recombinant protein reacted with both proteins. No reactivity to either recombinant protein was detected in nonimmune serum, and reactivity persisted as long as 20 weeks for one cat. The<jats:italic>p26<\/jats:italic>protein product is an immunodominant antigen that is expressed during infection in cats as a preprotein and is subsequently cleaved to form mature P26.<\/jats:p>","DOI":"10.1128\/cvi.00135-06","type":"journal-article","created":{"date-parts":[[2006,8,7]],"date-time":"2006-08-07T16:53:13Z","timestamp":1154969593000},"page":"830-836","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Cloning, Characterization, and Expression of<i>Bartonella henselae p26<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Jonathan A.","family":"Werner","sequence":"first","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunlian","family":"Feng","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rickie W.","family":"Kasten","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emir","family":"Hodzic","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno B.","family":"Chomel","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen W.","family":"Barthold","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine"},{"name":"Department of Population Health and Reproduction, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 95616"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0147-9571(96)00025-2","volume":"20","year":"1997","unstructured":"Abbott, R. 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