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Currently, symptomatic supportive treatment and fluid replacement therapy are the only means available to minimize DEN-induced mortality. As the clinical symptoms associated with DEN virus infections are indistinguishable from those of many other viral, bacterial, and parasitic infections, specific diagnostic tests assume critical importance in the unequivocal identification of DEN virus infections. We have designed a novel chimeric antigen based on envelope domain III (EDIII), a critical antigenic region of the major structural protein of DEN viruses. We fused EDIIIs corresponding to each of the four DEN virus serotypes using pentaglycyl linkers, overexpressed the resultant tetravalent chimeric protein in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , and affinity purified it in high yields, obtaining \u223c30 mg protein of &gt;95% purity per liter of culture. We show that this tetravalent antigen could specifically recognize anti-DEN virus antibodies of both the immunoglobulin M (IgM) and IgG classes. Using a large panel of IgM antibody capture-enzyme-linked immunosorbent assay- and hemagglutination inhibition-confirmed DEN virus-infected and uninfected patient sera (\n            <jats:italic>n<\/jats:italic>\n            = 289), we demonstrate that this tetravalent antigen can function as a diagnostic tool of high sensitivity and specificity.\n          <\/jats:p>","DOI":"10.1128\/cvi.00145-07","type":"journal-article","created":{"date-parts":[[2007,9,27]],"date-time":"2007-09-27T00:59:26Z","timestamp":1190854766000},"page":"1505-1514","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Single Antigen Detects both Immunoglobulin M (IgM) and IgG Antibodies Elicited by All Four Dengue Virus Serotypes"],"prefix":"10.1128","volume":"14","author":[{"given":"Menaka D.","family":"Hapugoda","sequence":"first","affiliation":[{"name":"Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India"},{"name":"Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaurav","family":"Batra","sequence":"additional","affiliation":[{"name":"Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India"},{"name":"Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Abeyewickreme","sequence":"additional","affiliation":[{"name":"Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India"},{"name":"Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Swaminathan","sequence":"additional","affiliation":[{"name":"Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India"},{"name":"Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Khanna","sequence":"additional","affiliation":[{"name":"Recombinant Gene Products Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India"},{"name":"Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.13.1.59-67.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.pep.2005.01.009","volume":"41","year":"2005","unstructured":"AnandaRao, R., S. 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