{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T10:15:58Z","timestamp":1768472158021,"version":"3.49.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Gold standard serological diagnostic methods focus on antigens that elicit a strong humoral immune response that is specific to a certain pathogen. In this study, we used bioinformatics approaches to identify linear B-cell epitopes that are conserved among<jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>species but are divergent from the host species<jats:named-content content-type=\"genus-species\">Homo sapiens<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Canis familiaris<\/jats:named-content>and from<jats:named-content content-type=\"genus-species\">Trypanosoma cruzi<\/jats:named-content>, the parasite that causes Chagas disease, to select potential targets for the immunodiagnosis of leishmaniasis. Using these criteria, we selected heat shock protein 83.1 of<jats:named-content content-type=\"genus-species\">Leishmania braziliensis<\/jats:named-content>for this study. We predicted three linear B-cell epitopes in its sequence. These peptides and the recombinant heat shock protein 83.1 (rHSP83.1) were tested in enzyme-linked immunosorbent assays (ELISAs) against serum samples from patients with tegumentary leishmaniasis (TL) and visceral leishmaniasis (VL) and from dogs infected with<jats:named-content content-type=\"genus-species\">Leishmania infantum<\/jats:named-content>(canine VL [CVL]). Our data show that rHSP83.1 is a promising target in the diagnosis of TL. We also identified specific epitopes derived from HSP83.1 that can be used in the diagnosis of human TL (peptide 3), both human and canine VL (peptides 1 and 3), and all TL, VL, and CVL clinical manifestations (peptide 3). Receiver operating characteristic (ROC) curves confirmed the superior performance of rHSP83.1 and peptides 1 and 3 compared to that of the soluble<jats:named-content content-type=\"genus-species\">L. braziliensis<\/jats:named-content>antigen and the reference test kit for the diagnosis of CVL in Brazil (EIE-LVC kit; Bio-Manguinhos, Fiocruz). Our study thus provides proof-of-principle evidence of the feasibility of using bioinformatics to identify novel targets for the immunodiagnosis of parasitic diseases using proteins that are highly conserved throughout evolution.<\/jats:p>","DOI":"10.1128\/cvi.00151-14","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:28:50Z","timestamp":1399508930000},"page":"949-959","source":"Crossref","is-referenced-by-count":27,"title":["Epitope Mapping of the HSP83.1 Protein of Leishmania braziliensis Discloses Novel Targets for Immunodiagnosis of Tegumentary and Visceral Clinical Forms of Leishmaniasis"],"prefix":"10.1128","volume":"21","author":[{"given":"Daniel","family":"Menezes-Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago Ant\u00f4nio de Oliveira","family":"Mendes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus de Souza","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Lu\u00eds","family":"Reis-Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronaldo Alves Pinto","family":"Nagem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Martins","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo Ant\u00f4nio Ferraz","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00facia Maria da Cunha","family":"Galv\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Toshio","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella Castanheira","family":"Bartholomeu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","published-online":{"date-parts":[[2014,5,7]]},"reference":[{"key":"key20180919073737_B1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1007\/s004300100085","article-title":"Worldwide increasing risk factors for leishmaniasis","volume":"190","author":"Desjeux","year":"2001","journal-title":"Med. 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