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In this work, we have identified epitopes within the<jats:italic>Giardia<\/jats:italic>variant-specific surface protein (VSP) sequences that are recognized by IgG antibodies from 13 of 14 (93%) sera from patients with stool-confirmed giardiasis. The conserved epitopes are shared among VSPs from both of the assemblages that commonly infect humans, and they are likely to be structural, as both sodium dodecyl sulfate treatment and dithiothreitol reduction decrease antibody recognition. In a multiplex bead assay (MBA), we used three VSP fragments from an assemblage A<jats:italic>Giardia<\/jats:italic>strain, three VSP fragments from assemblage B strains, and the \u03b1-1 giardin structural antigen to detect IgG antibodies to<jats:italic>Giardia<\/jats:italic>and used the recombinant 17- and 27-kDa antigens to simultaneously detect IgG antibodies to<jats:italic>Cryptosporidium<\/jats:italic>. The MBA differentiated between sera from<jats:italic>Giardia<\/jats:italic>and<jats:italic>Cryptosporidium<\/jats:italic>outbreaks and also identified a giardiasis outbreak that may have included cryptosporidiosis cases. Approximately 40% of cryptosporidiosis outbreak samples had high MBA responses for both the 27- and 17-kDa antigens, while &lt;10% of nonoutbreak and giardiasis outbreak samples had high responses. At least 60% of giardiasis outbreak samples were positive for antibodies to multiple<jats:italic>Giardia<\/jats:italic>antigens, while \u226412% of nonoutbreak samples and samples from U.S. and British Columbia cryptosporidiosis outbreaks met our definition for<jats:italic>Giardia<\/jats:italic>seropositivity. A MBA using multiple parasite antigens may prove useful in the epidemiologic analysis of future waterborne or food-borne outbreaks of diarrheal disease.<\/jats:p>","DOI":"10.1128\/cvi.00160-10","type":"journal-article","created":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T03:39:34Z","timestamp":1285731574000},"page":"1695-1707","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize<i>Giardia intestinalis<\/i>and<i>Cryptosporidium parvum<\/i>Antigens"],"prefix":"10.1128","volume":"17","author":[{"given":"Jeffrey W.","family":"Priest","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delynn M.","family":"Moss","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Govinda S.","family":"Visvesvara","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara C.","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith L.","family":"Isaac-Renton","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"},{"name":"Atlanta Research and Education Foundation, Decatur, Georgia"},{"name":"Department of Pathology and Laboratory Medicine and Department of Health Care and Epidemiology, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.4269\/ajtmh.1991.44.63","volume":"44","year":"1991","unstructured":"Abaza, S. 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