{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:47:46Z","timestamp":1761155266962},"reference-count":27,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.<\/jats:p>","DOI":"10.1128\/cvi.00163-09","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T02:01:21Z","timestamp":1245895281000},"page":"1132-1138","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused by South American Arenaviruses"],"prefix":"10.1128","volume":"16","author":[{"given":"Mina","family":"Nakauchi","sequence":"first","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuetsu","family":"Fukushi","sequence":"additional","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Saijo","sequence":"additional","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agusti\u0301n E.","family":"Ure","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas, Instituto de Biotecnologi\u0301a y Biologi\u0301a Molecular, Universidad Nacional de La Plata, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBBM-UNLP-CONICET-CCT La Plata), La Plata, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Romanowski","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas, Instituto de Biotecnologi\u0301a y Biologi\u0301a Molecular, Universidad Nacional de La Plata, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBBM-UNLP-CONICET-CCT La Plata), La Plata, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kurane","sequence":"additional","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[{"name":"Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0166-0934(92)90142-Z","volume":"39","year":"1992","unstructured":"Bockstahler, L. 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