{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:19:57Z","timestamp":1781583597842,"version":"3.54.5"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Brucella abortus<\/jats:named-content>readily multiplies in professional or nonprofessional phagocytes<jats:italic>in vitro<\/jats:italic>and is highly virulent in mice. Isogenic mutants of<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>biovar 1 strain IVKB9007 lacking the ATP\/GDP-binding protein motif A (P-loop) (named<jats:italic>looP<\/jats:italic>; designated here the IVKB9007<jats:italic>looP<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>mutant) and the ATP-binding\/permease protein (<jats:italic>cydC<\/jats:italic>; designated here the IVKB9007<jats:italic>cydC<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>mutant) were identified and characterized by transposon mutagenesis using the mini-Tn<jats:italic>5<\/jats:italic>Km2 transposon. Both mutants were found to be virtually incapable of intracellular replication in both murine macrophages (RAW264.7) and the HeLa cell line, and their virulence was significantly impaired in BALB\/c mice. Respective complementation of the IVKB9007<jats:italic>looP<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>and IVKB9007<jats:italic>cydC<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>mutants restored their ability to survive<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>to a level comparable with that of the wild type. These findings indicate that the<jats:italic>cydC<\/jats:italic>and<jats:italic>looP<\/jats:italic>genes play important roles in the virulence of<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>. In addition, intraperitoneal immunization of mice with a dose of the live IVKB9007<jats:italic>looP<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>and IVKB9007<jats:italic>cydC<\/jats:italic>::Tn<jats:italic>5<\/jats:italic>mutants provided a high degree of protection against challenge with pathogenic<jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>strain 544. Both mutants should be evaluated further as a live attenuated vaccine against bovine brucellosis for their ability to stimulate a protective immune response.<\/jats:p>","DOI":"10.1128\/cvi.00164-14","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T07:00:24Z","timestamp":1411628424000},"page":"1573-1580","source":"Crossref","is-referenced-by-count":17,"title":["Characterization and Protective Property of Brucella abortus<i>cydC<\/i>and<i>looP<\/i>Mutants"],"prefix":"10.1128","volume":"21","author":[{"given":"Quang Lam","family":"Truong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngjae","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhijit Kashinath","family":"Barate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suk","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Wook","family":"Hahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2014,9,24]]},"reference":[{"key":"key20180919073750_B1","doi-asserted-by":"publisher","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. 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