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Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as<jats:italic>Lactobacillus<\/jats:italic>spp. In this study, we used the Lyme disease mouse model as a proof of concept. We demonstrate that an oral vaccine based on live recombinant<jats:italic>Lactobacillus plantarum<\/jats:italic>protects mice from tick-transmitted<jats:italic>Borrelia burgdorferi<\/jats:italic>infection. Our method of expressing vaccine antigens in<jats:italic>L. plantarum<\/jats:italic>induces both systemic and mucosal immunity after oral administration. This platform technology can be applied to design oral vaccine delivery vehicles against several microbial pathogens.<\/jats:p>","DOI":"10.1128\/cvi.00169-08","type":"journal-article","created":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T01:39:58Z","timestamp":1216258798000},"page":"1429-1435","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Oral Immunization with Recombinant<i>Lactobacillus plantarum<\/i>Induces a Protective Immune Response in Mice with Lyme Disease"],"prefix":"10.1128","volume":"15","author":[{"given":"Beatriz","family":"del Rio","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York"},{"name":"Biopeptides Corp., Valhalla, New York"},{"name":"Falco Biotherapeutics BV, Zernikedreef 9, 2331 CK Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond J.","family":"Dattwyler","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York"},{"name":"Biopeptides Corp., Valhalla, New York"},{"name":"Falco Biotherapeutics BV, Zernikedreef 9, 2331 CK Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Aroso","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York"},{"name":"Biopeptides Corp., Valhalla, New York"},{"name":"Falco Biotherapeutics BV, Zernikedreef 9, 2331 CK Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Neves","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York"},{"name":"Biopeptides Corp., Valhalla, New York"},{"name":"Falco Biotherapeutics BV, Zernikedreef 9, 2331 CK Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, New York Medical College, Valhalla, New York"},{"name":"Biopeptides Corp., Valhalla, New York"},{"name":"Falco Biotherapeutics BV, Zernikedreef 9, 2331 CK Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos F. 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