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It is postulated that CD8<jats:sup>+<\/jats:sup>T cell responses play an important role in controlling HHV-8 infection and preventing development of disease. In this study, we investigated monofunctional and polyfunctional CD8<jats:sup>+<\/jats:sup>T cell responses to HHV-8 lytic proteins gB (glycoprotein B) and K8.1 and latency proteins LANA-1 (latency-associated nuclear antigen-1) and K12. On the basis of our previous findings that dendritic cells (DC) reveal major histocompatibility complex (MHC) class I epitopes in gB, we used a DC-based system to identify 2 novel epitopes in gB, 2 in K8.1, 5 in LANA-1, and 1 in K12. These new HHV-8 epitopes activated monofunctional and polyfunctional CD8<jats:sup>+<\/jats:sup>T cells that produced various combinations of gamma interferon, interleukin 2, tumor necrosis factor alpha, macrophage inhibitory protein 1\u03b2, and cytotoxic degranulation marker CD107a in healthy HHV-8-seropositive individuals. We were also able to detect HHV-8-specific CD8<jats:sup>+<\/jats:sup>T cells in peripheral blood samples using HLA A*0201 pentamer complexes for one gB epitope, one K8.1 epitope, two LANA-1 epitopes, and one K12 epitope. These immunogenic regions of viral lytic and latency proteins could be important in T cell control of HHV-8 infection.<\/jats:p>","DOI":"10.1128\/cvi.00189-10","type":"journal-article","created":{"date-parts":[[2010,8,19]],"date-time":"2010-08-19T02:04:20Z","timestamp":1282183460000},"page":"1507-1516","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Monofunctional and Polyfunctional CD8<sup>+<\/sup>T Cell Responses to Human Herpesvirus 8 Lytic and Latency Proteins"],"prefix":"10.1128","volume":"17","author":[{"given":"Lauren","family":"Lepone","sequence":"first","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Rappocciolo","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilee","family":"Knowlton","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariel","family":"Jais","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Piazza","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank J.","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Rinaldo","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health"},{"name":"School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4818"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1097\/QAD.0b013e3283300a91","volume":"23","year":"2009","unstructured":"Bihl, F., C. 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