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BALB\/c mice were subcutaneously or intraperitoneally immunized with a single dose or three doses of the<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>\u0394<jats:italic>abcBA<\/jats:italic>strain and then were challenged with wild-type<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>. Single or multiple immunizations provided only mild protection, with significantly smaller numbers of wild-type<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>CFU in the livers of immunized mice but not in the spleens. Encapsulation of<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>\u0394<jats:italic>abcBA<\/jats:italic>significantly improved protection against experimental challenges in both BALB\/c and C57BL\/6 mice. Furthermore, immunization with encapsulated<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>\u0394<jats:italic>abcBA<\/jats:italic>markedly prevented lesions in the spleens and livers of experimentally challenged mice. These results demonstrated that the encapsulated<jats:named-content content-type=\"genus-species\">B. ovis<\/jats:named-content>\u0394<jats:italic>abcBA<\/jats:italic>strain confers protection to mice; therefore, this strain has potential as a vaccine candidate for rams.<\/jats:p>","DOI":"10.1128\/cvi.00191-15","type":"journal-article","created":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T02:01:01Z","timestamp":1430964061000},"page":"789-797","source":"Crossref","is-referenced-by-count":26,"title":["Protection Provided by an Encapsulated Live Attenuated \u0394<i>abcBA<\/i>Strain of Brucella ovis against Experimental Challenge in a Murine Model"],"prefix":"10.1128","volume":"22","author":[{"given":"Ana Patr\u00edcia C.","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auric\u00e9lio A.","family":"Mac\u00eado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teane M. 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