{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T20:17:20Z","timestamp":1781554640369,"version":"3.54.5"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin. Vaccine Immunol."],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We describe a retrospective analysis of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>enzyme immunoassay (EIA) IgM and IgG results compared to those of the standard tube agglutination test (SAT). Among 1,091 samples tested, 104 (9.5%) and 24 (2.2%) sera were positive by IgM and IgG EIA, respectively. Supplemental testing by SAT showed that 82.7% (86\/104) of IgM EIA-reactive samples and 54.2% (13\/24) of IgG EIA-reactive samples were negative by SAT. Testing all EIA screen-reactive samples by SAT is required when evaluating patients for potential brucellosis. Due to the limitations of serology, culture remains the gold standard for detecting<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>infection.<\/jats:p>","DOI":"10.1128\/cvi.00197-12","type":"journal-article","created":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T13:53:28Z","timestamp":1340286808000},"page":"1332-1334","source":"Crossref","is-referenced-by-count":12,"title":["A High Percentage of Serum Samples That Test Reactive by Enzyme Immunoassay for Anti-Brucella Antibodies Are Not Confirmed by the Standard Tube Agglutination Test"],"prefix":"10.1128","volume":"19","author":[{"given":"Matthew J.","family":"Binnicker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elitza S.","family":"Theel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Staci M.","family":"Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2012,6,20]]},"reference":[{"key":"key20180919073619_B1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jiph.2012.02.001","article-title":"Serological and molecular diagnosis of human brucellosis in Najran, Southwestern Saudi Arabia","volume":"5","author":"Asaad","year":"2012","journal-title":"J. Infect. Public Health"},{"key":"key20180919073619_B2","unstructured":"CDC. 2007, posting date. Brucellosis, general information. Department of Health and Human Services, Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/www.cdc.gov\/ncidod\/dbmd\/diseaseinfo\/brucellosis_g.htm."},{"key":"key20180919073619_B3","first-page":"603","article-title":"Public health consequences of a false-positive laboratory test result for Brucella\u2014Florida, Georgia, and Michigan, 2005","volume":"57","author":"CDC","year":"2008","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"key20180919073619_B4","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0732-8893(02)00426-1","article-title":"Comparison of the Brucella standard agglutination test with the ELISA IgG and IgM in patients with Brucella bacteremia","volume":"44","author":"Memish","year":"2002","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"key20180919073619_B5","doi-asserted-by":"crossref","first-page":"272","DOI":"10.7883\/yoken.64.272","article-title":"Evaluation of serological tests for diagnosis of brucellosis","volume":"64","author":"Pabuccuoglu","year":"2011","journal-title":"Jpn. J. Infect. Dis."},{"key":"key20180919073619_B6","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.diagmicrobio.2009.06.002","article-title":"Performance characteristics of the Euroimmun enzyme-linked immunosorbent assay kits for Brucella IgG and IgM","volume":"65","author":"Prince","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"key20180919073619_B7","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.jhin.2011.12.020","article-title":"Laboratory-acquired brucellosis in Turkey","volume":"80","author":"Sayin-Kutlu","year":"2012","journal-title":"J. Hosp. Infect."},{"key":"key20180919073619_B8","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1089\/vbz.2011.0704","article-title":"Limitations of the standard agglutination test for detecting patients with Brucella melitensis bacteremia","volume":"12","author":"Shemesh","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"key20180919073619_B9","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/jcla.20382","article-title":"A comparison of Brucella IgG and IgM ELISA assays with agglutination methodology","volume":"24","author":"Welch","year":"2010","journal-title":"J. Clin. Lab. Anal."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1128\/CVI.00197-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T20:35:10Z","timestamp":1687552510000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00197-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,20]]},"references-count":9,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2012,7,31]]},"published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1128\/CVI.00197-12"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00197-12","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,20]]}}}