{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:19:43Z","timestamp":1791908383256,"version":"4.3.4"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The ability of a recombinant\n            <jats:italic>Mycobacterium bovis<\/jats:italic>\n            BCG strain that secretes major membrane protein II (MMP-II) of\n            <jats:italic>Mycobacterium leprae<\/jats:italic>\n            (BCG-SM) to confer protection against leprosy was evaluated by use of a mouse footpad model. C57BL\/6J mice intradermally inoculated with BCG-SM produced splenic T cells which secreted significant amounts of gamma interferon (IFN-\u03b3) in response to either the recombinant MMP-II, the\n            <jats:italic>M. leprae<\/jats:italic>\n            -derived membrane fraction, or the BCG-derived cytosolic fraction in vitro more efficiently than those from the mice infected with the vector control BCG strain (BCG-pMV, a BCG strain containing pMV-261). A higher percentage of CD8\n            <jats:sup>+<\/jats:sup>\n            T cells obtained from BCG-SM-inoculated mice than those obtained from BCG-pMV-inoculated mice produced intracellular IFN-\u03b3 on restimulation with the\n            <jats:italic>M. leprae<\/jats:italic>\n            antigens. BCG-SM inhibited the multiplication of\n            <jats:italic>M. leprae<\/jats:italic>\n            in the footpads of C57BL\/6J mice more efficiently than BCG-pMV. These results indicate that a BCG strain that secretes MMP-II could be a better vaccine candidate for leprosy.\n          <\/jats:p>","DOI":"10.1128\/cvi.00203-09","type":"journal-article","created":{"date-parts":[[2009,8,13]],"date-time":"2009-08-13T02:04:37Z","timestamp":1250129077000},"page":"1399-1404","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of the Multiplication of\n            <i>Mycobacterium leprae<\/i>\n            by Vaccination with a Recombinant\n            <i>M. bovis<\/i>\n            BCG Strain That Secretes Major Membrane Protein II in Mice"],"prefix":"10.1128","volume":"16","author":[{"given":"Y.","family":"Maeda","sequence":"first","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Makino","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"59","volume":"67","year":"1999","unstructured":"Cho, S. 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