{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:11:25Z","timestamp":1790745085736,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The similarity of endospore surface antigens between bacteria of the\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            group complicates the development of selective antibody-based anthrax detection systems. The surface of\n            <jats:italic>B. anthracis<\/jats:italic>\n            endospores exposes a tetrasaccharide containing the monosaccharide anthrose. Anti-tetrasaccharide monoclonal antibodies (MAbs) and anti-anthrose-rhamnose disaccharide MAbs were produced and tested for their fine specificities in a direct spore enzyme-linked immunosorbent assay (ELISA) with inactivated spores of a broad spectrum of\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains and related species of the\n            <jats:italic>Bacillus<\/jats:italic>\n            genus. Although the two sets of MAbs had different fine specificities, all of them recognized the tested\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains and showed only a limited cross-reactivity with two\n            <jats:italic>B. cereus<\/jats:italic>\n            strains. The MAbs were further tested for their ability to be implemented in a highly sensitive and specific bead-based Luminex assay. This assay detected spores from different\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains and two cross-reactive\n            <jats:italic>B. cereus<\/jats:italic>\n            strains, correlating with the results obtained in direct spore ELISA. The Luminex assay (detection limit 10\n            <jats:sup>3<\/jats:sup>\n            to 10\n            <jats:sup>4<\/jats:sup>\n            spores per ml) was much more sensitive than the corresponding sandwich ELISA. Although not strictly specific for\n            <jats:italic>B. anthracis<\/jats:italic>\n            spores, the developed Luminex assay represents a useful first-line screening tool for the detection of\n            <jats:italic>B. anthracis<\/jats:italic>\n            spores.\n          <\/jats:p>","DOI":"10.1128\/cvi.00205-10","type":"journal-article","created":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T02:20:00Z","timestamp":1279765200000},"page":"1446-1451","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Anthrax Spore Detection by a Luminex Assay Based on Monoclonal Antibodies That Recognize Anthrose-Containing Oligosaccharides"],"prefix":"10.1128","volume":"17","author":[{"given":"Marco","family":"Tamborrini","sequence":"first","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstr. 57, CH 4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH 4003 Basel, Switzerland"},{"name":"Federal Office for Civil Protection, Spiez Laboratory, CH 3700 Spiez, Switzerland"},{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Mu\u0308hlenberg 1, D 14476 Potsdam, Germany"},{"name":"Free University Berlin, Institute for Chemistry and Biochemistry, Arnimallee 22, D 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelle","family":"Holzer","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstr. 57, CH 4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH 4003 Basel, Switzerland"},{"name":"Federal Office for Civil Protection, Spiez Laboratory, CH 3700 Spiez, Switzerland"},{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Mu\u0308hlenberg 1, D 14476 Potsdam, Germany"},{"name":"Free University Berlin, Institute for Chemistry and Biochemistry, Arnimallee 22, D 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter H.","family":"Seeberger","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstr. 57, CH 4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH 4003 Basel, Switzerland"},{"name":"Federal Office for Civil Protection, Spiez Laboratory, CH 3700 Spiez, Switzerland"},{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Mu\u0308hlenberg 1, D 14476 Potsdam, Germany"},{"name":"Free University Berlin, Institute for Chemistry and Biochemistry, Arnimallee 22, D 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Schu\u0308rch","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstr. 57, CH 4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH 4003 Basel, Switzerland"},{"name":"Federal Office for Civil Protection, Spiez Laboratory, CH 3700 Spiez, Switzerland"},{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Mu\u0308hlenberg 1, D 14476 Potsdam, Germany"},{"name":"Free University Berlin, Institute for Chemistry and Biochemistry, Arnimallee 22, D 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Pluschke","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute, Socinstr. 57, CH 4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH 4003 Basel, Switzerland"},{"name":"Federal Office for Civil Protection, Spiez Laboratory, CH 3700 Spiez, Switzerland"},{"name":"Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Am Mu\u0308hlenberg 1, D 14476 Potsdam, Germany"},{"name":"Free University Berlin, Institute for Chemistry and Biochemistry, Arnimallee 22, D 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1016\/j.carres.2005.09.015","volume":"340","year":"2005","unstructured":"Adamo, R., R. Saksena, and P. Kov\u00e1\u010d. 2005. Synthesis of the \u03b2 anomer of the spacer-equipped tetrasaccharide side chain of the major glycoprotein of the Bacillus anthracis exosporium. Carbohydr. Res.340:2579-2582.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_3_2","first-page":"850","volume":"4","year":"2003","unstructured":"Andreotti, P. E., G. V. Ludwig, A. H. Peruski, J. J. Tuite, S. S. Morse, and L. F. Peruski, Jr. 2003. Immunoassay of infectious agents. Biotechniques4:850-859.","journal-title":"Biotechniques"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.tvjl.2004.04.021","volume":"170","year":"2005","unstructured":"Berry, J. D. 2005. Rational monoclonal antibody development to emerging pathogens, biothreat agents and agents of foreign animal disease: the antigen scale. Vet. J.170:193-211.","journal-title":"Vet. J."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"6513","DOI":"10.1021\/jo070750s","volume":"72","year":"2007","unstructured":"Crich, D., and O. Vinogradova. 2007. Synthesis of the antigenic tetrasaccharide side chain from the major glycoprotein of Bacillus anthracis exosporium. J. Org. Chem.72:6513-6520.","journal-title":"J. Org. Chem."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.8.3665-3670.2001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"30945","DOI":"10.1074\/jbc.M401613200","volume":"279","year":"2004","unstructured":"Daubenspeck, J. M., H. Zeng, P. Chen, S. Dong, C. T. Steichen, N. R. Krishna, D. G. Pritchard, and C. L. Turnbough, Jr. 2004. Novel oligosaccharide side chains of the collagen-like region of BclA, the major glycoprotein of the Bacillus anthracis exosporium. J. Biol. Chem.279:30945-30953.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01899-07"},{"key":"e_1_3_2_9_2","first-page":"1504","volume":"5","year":"1967","unstructured":"Gerhardt, P. 1967. Cytology of Bacillus anthracis. Fed. Proc.5:1504-1517.","journal-title":"Fed. Proc."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"5206","DOI":"10.1002\/anie.200701354","volume":"46","year":"2007","unstructured":"Guo, H., and G. A. O'Doherty. 2007. De novo asymmetric synthesis of the anthrax tetrasaccharide by a palladium-catalyzed glycosylation reaction. Angew. Chem. Int. Ed. Engl.46:5206-5208.","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2627-2630.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.forsciint.2006.09.004","volume":"171","year":"2007","unstructured":"Hoile, R., M. Yuen, G. James, and G. L. Gilbert. 2007. Evaluation of the rapid analyte measurement platform (RAMP) for the detection of Bacillus anthracis at a crime scene. Forensic Sci. Int.171:1-4.","journal-title":"Forensic Sci. Int."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3454-3455.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"8709","DOI":"10.1073\/pnas.0803897105","volume":"105","year":"2008","unstructured":"Kubler-Kielb, J., E. Vinogradov, H. Hu, S. H. Leppla, J. B. Robbins, and R. Schneerson. 2008. Saccharides cross-reactive with Bacillus anthracis spore glycoprotein as an anthrax vaccine component. Proc. Natl. Acad. Sci. U. S. A.105:8709-8712.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00235-09"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"9136","DOI":"10.1002\/chem.200601245","volume":"12","year":"2006","unstructured":"Mehta, A. S., E. Saile, W. Zhong, T. Buskas, R. Carlson, E. Kannenberg, Y. Reed, C. P. Quinn, and G. J. Boons. 2006. Synthesis and antigenic analysis of the BclA glycoprotein oligosaccharide from the Bacillus anthracis exosporium. Chemistry12:9136-9149.","journal-title":"Chemistry"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0022-1759(98)00041-6","volume":"215","year":"1998","unstructured":"Pluschke, G., A. Joss, J. Marfurt, C. Daubenberger, O. Kashala, M. Zwickl, A. Stief, G. Sansig, B. Schlapfer, S. Linkert, H. van der Putten, N. Hardman, and M. Schroder. 1998. Generation of chimeric monoclonal antibodies from mice that carry human immunoglobulin Cgamma1 heavy of Ckappa light chain gene segments. J. Immunol. Methods215:27-37.","journal-title":"J. Immunol. Methods"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/j.carres.2005.04.010","volume":"340","year":"2005","unstructured":"Saksena, R., R. Adamo, and P. Kov\u00e1\u010d. 2005. Studies toward a conjugate vaccine for anthrax. Synthesis and characterization of anthrose [4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methyl-d-glucopyranose] and its methyl glycosides. Carbohydr. Res.340:1591-1600.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.bmcl.2005.10.056","volume":"16","year":"2006","unstructured":"Saksena, R., R. Adamo, and P. Kov\u00e1\u010d. 2006. Synthesis of the tetrasaccharide side chain of the major glycoprotein of the Bacillus anthracis exosporium. Bioorg. Med. Chem. Lett.16:615-617.","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"4283","DOI":"10.1016\/j.bmc.2007.03.057","volume":"15","year":"2007","unstructured":"Saksena, R., R. Adamo, and P. Kov\u00e1\u010d. 2007. Immunogens related to the synthetic tetrasaccharide side chain of Bacillus anthracis exosporium. Bioorg. Med. Chem. Lett.15:4283-4310.","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1111\/j.1365-2672.2008.04129.x","volume":"106","year":"2009","unstructured":"Tamborrini, M., M. A. Oberli, D. B. Werz, N. Sch\u00fcrch, J. Frey, P. H. Seeberger, and G. Pluschke. 2009. Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains. J. Appl. Microbiol.106:1618-1628.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"6581","DOI":"10.1002\/anie.200602048","volume":"45","year":"2006","unstructured":"Tamborrini, M., D. B. Werz, J. Frey, G. Pluschke, and P. H. Seeberger. 2006. Anti-carbohydrate antibodies for the detection of anthrax spores. Angew. Chem. Int. Ed. Engl.45:6581-6582.","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"e_1_3_2_23_2","first-page":"1976","volume":"12","year":"2007","unstructured":"Werz, D. B., A. Adibekian, and P. H. Seeberger. 2007. Synthesis of a spore surface pentasaccharide of Bacillus anthracis. Eur. J. Org. Chem.12:1976-1982.","journal-title":"Eur. J. Org. Chem."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"6315","DOI":"10.1002\/anie.200502615","volume":"44","year":"2005","unstructured":"Werz, D. B., and P. H. Seeberger. 2005. Total synthesis of antigen Bacillus anthracis tetrasaccharide\u2014creation of an anthrax vaccine candidate. Angew. Chem. Int. Ed. Engl.44:6315-6318.","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"e_1_3_2_25_2","unstructured":"Guidelines for the surveillance and control of anthrax in humans and animals 1998"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00205-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00205-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T20:44:10Z","timestamp":1627591450000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00205-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1128\/CVI.00205-10"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00205-10","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]},"assertion":[{"value":"2010-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}