{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:44:40Z","timestamp":1759009480384},"reference-count":23,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Variations in class 2\/3 (PorB) proteins form the basis for meningococcal serotyping. Antibodies against these proteins are bactericidal, making serotyping results useful not only for epidemiological surveillance of meningococcal disease but also for identifying potential vaccine components. A total of 20 to 60% of meningococcal B and C isolates from any given population are nontypeable (NT) using a panel of monoclonal antibodies. To analyze the mechanisms responsible for the nonserotypeability characteristic in\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            , we (i) established the nucleotide sequences of\n            <jats:italic>porB<\/jats:italic>\n            gene in 146 meningococcal strains (95 not recognized by the serotyping panel), (ii) identified 18 new allelic variants of the\n            <jats:italic>porB<\/jats:italic>\n            gene, (iii) correlated allelic variants with serotypes, (iv) suggest the nontypeability characteristic in those 95 NT strains, and (v) reject the possibility of variation in the levels of PorB expression.\n          <\/jats:p>","DOI":"10.1128\/cvi.00211-06","type":"journal-article","created":{"date-parts":[[2006,10,6]],"date-time":"2006-10-06T20:24:23Z","timestamp":1160166263000},"page":"1087-1091","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Sequencing of the\n            <i>porB<\/i>\n            Gene: a Step toward a True Characterization of\n            <i>Neisseria meningitidis<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"R.","family":"Abad","sequence":"first","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Alcala\u0301","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Salcedo","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Enri\u0301quez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Uri\u0301a","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Diez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Va\u0301zquez","sequence":"additional","affiliation":[{"name":"Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"177","volume":"26","year":"1998","unstructured":"Abdillahi, H., and J. T. Poolman. 1998. Typing of group B Neisseria meningitidis with monoclonal antibodies in the whole-cell ELISA. J. Med. Microbiol.26:177-180.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1099\/jmm.0.05517-0","volume":"53","year":"2004","unstructured":"Alcal\u00e1, B., C. Salcedo, L. Arreaza, R. Abad, R. Enr\u00edquez, L. de la Fuente, M. J. Ur\u00eda, and J. A. V\u00e1zquez. 2004. Antigenic and\/or phase variation of PorA protein in non-subtypeable Neisseria meningitidis strains isolated in Spain. J. Med. Microbiol.53:515-518.","journal-title":"J. Med. 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