{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:03:01Z","timestamp":1759935781147,"version":"3.38.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Previously, we showed that surface protective antigen (Spa) proteins of<jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>can be classified into three molecular species\u2014SpaA, SpaB, and SpaC\u2014and that SpaC is the most broadly cross-protective antigen among the three Spa proteins. In this study, we examined the ability of the \u03b1-helical domain, which comprises the N-terminal half of SpaC, to elicit cross-protective immunity in mice and pigs. Mice actively immunized with the full-length protein (rSpaC664) or the \u03b1-helical domain (rSpaC427), but not the C-terminal domain (rSpaC253), were protected against challenge with<jats:italic>E. rhusiopathiae<\/jats:italic>serovars 1a, 2, 6, 19, and 18 expressing heterologous (SpaA or SpaB) and homologous (SpaC) Spas. The \u03b1-helical domain seemed to provide better protection than rSpaC664, although the differences did not reach statistical significance. Similarly, mice passively immunized with rabbit anti-rSpaC664 or anti-rSpaC427 sera, but not anti-rSpaC253 serum, were protected from challenge with various serovars. Pigs immunized with SpaC427 also developed specific antibodies against Spa proteins and were protected from challenge with the highly virulent heterologous<jats:italic>E. rhusiopathiae<\/jats:italic>strain Fujisawa (serovar 1a). Taken together, these results demonstrate for the first time the striking protective efficacy of the \u03b1-helical domain-mediated immunization in both mice and pigs, thereby highlighting its utility as the most promising candidate for the development of a safe and effective vaccine against erysipelas.<\/jats:p>","DOI":"10.1128\/cvi.00213-10","type":"journal-article","created":{"date-parts":[[2010,10,7]],"date-time":"2010-10-07T02:40:05Z","timestamp":1286419205000},"page":"1991-1997","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Immunization with Truncated Recombinant Protein SpaC of<i>Erysipelothrix rhusiopathiae<\/i>Strain 715 Serovar 18 Confers Protective Immunity against Challenge with Various Serovars"],"prefix":"10.1128","volume":"17","author":[{"given":"Ho","family":"To","sequence":"first","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichi","family":"Someno","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Nagai","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Koyama","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuji","family":"Nagano","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shinmachi, Ome, Tokyo 198-0024, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6533-6542.1999"},{"key":"e_1_3_2_3_2","first-page":"589","volume":"25","year":"1964","unstructured":"Freeman, M. 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