{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T05:46:55Z","timestamp":1750139215109},"reference-count":49,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of this study was to evaluate the protective efficacy of MTBK_24820, a complete form of PPE39 protein derived from a predominant Beijing\/K strain of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            in South Korea. Mice were immunized with MTKB_24820,\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            Bacilli Calmette-Gu\u00e9rin (BCG), or adjuvant prior to a high-dosed Beijing\/K strain aerosol infection. After 4 and 9 weeks, bacterial loads were determined and histopathologic and immunologic features in the lungs and spleens of the\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            -infected mice were analyzed. Putative immunogenic T-cell epitopes were examined using synthetic overlapping peptides. Successful immunization of MTBK_24820 in mice was confirmed by increased IgG responses (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05) and recalled gamma interferon (IFN-\u03b3), interleukin-2 (IL-2), IL-6, and IL-17 responses (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05 or\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01) to MTBK_24820. After challenge with the Beijing\/K strain, an approximately 0.5 to 1.0 log\n            <jats:sub>10<\/jats:sub>\n            reduction in CFU in lungs and fewer lung inflammation lesions were observed in MTBK_24820-immunized mice compared to those for control mice. Moreover, MTBK_24820 immunization elicited significantly higher numbers of CD4\n            <jats:sup>+<\/jats:sup>\n            T cells producing protective cytokines, such as IFN-\u03b3 and IL-17, in lungs and spleens (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01) and CD4\n            <jats:sup>+<\/jats:sup>\n            multifunctional T cells producing IFN-\u03b3, tumor necrosis factor alpha (TNF-\u03b1), and\/or IL-17 (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01) than in control mice, suggesting protection comparable to that of BCG against the hypervirulent Beijing\/K strain. The dominant immunogenic T-cell epitopes that induced IFN-\u03b3 production were at the N terminus (amino acids 85 to 102 and 217 to 234). Its vaccine potential, along with protective immune responses\n            <jats:italic>in vivo<\/jats:italic>\n            , may be informative for vaccine development, particularly in regions where the\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            Beijing\/K-strain is frequently isolated from TB patients.\n          <\/jats:p>","DOI":"10.1128\/cvi.00219-17","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T03:15:33Z","timestamp":1504754133000},"update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing\/K Strain in Mice"],"prefix":"10.1128","volume":"24","author":[{"given":"Ahreum","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Gyoung","family":"Hur","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunwha","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Nae","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Global tuberculosis report 2016","author":"World Health Organization","unstructured":"World Health Organization. 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