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A capripoxvirus (CPV) recombinant virus (rKS1\/RVFV) was developed, which expressed the Rift Valley fever virus (RVFV) Gn and Gc glycoproteins. These expressed glycoproteins had the correct size and reacted with monoclonal antibodies (MAb) to native glycoproteins. Mice vaccinated with rKS1\/RVFV were protected against RVFV challenge. Sheep vaccinated with rKS1\/RVFV twice developed neutralizing antibodies and were significantly protected against RVFV and sheep poxvirus challenge. These findings further document the value of CPV recombinants as ruminant vaccine vectors and support the inclusion of RVFV genes encoding glycoproteins in multivalent recombinant vaccines to be used where RVF occurs.<\/jats:p>","DOI":"10.1128\/cvi.00220-10","type":"journal-article","created":{"date-parts":[[2010,9,29]],"date-time":"2010-09-29T03:39:34Z","timestamp":1285731574000},"page":"1842-1849","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Protection of Sheep against Rift Valley Fever Virus and Sheep Poxvirus with a Recombinant Capripoxvirus Vaccine"],"prefix":"10.1128","volume":"17","author":[{"given":"Reuben K.","family":"Soi","sequence":"first","affiliation":[{"name":"Kenya Agricultural Research Institute, P.O. Box 57811-00200, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred R.","family":"Rurangirwa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis C.","family":"McGuire","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Rwambo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1619"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"DeMartini","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523-1619"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy B.","family":"Crawford","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1016\/S0264-410X(02)00203-7","volume":"20","year":"2002","unstructured":"Aspden, K., A. 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