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The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>and challenge with a virulent<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (<jats:named-content content-type=\"genus-species\">Sus scrofa<\/jats:named-content>), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for<jats:italic>in vivo<\/jats:italic>TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-\u03b2), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>challenge. This information is relevant for pig production in regions that are endemic for<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>and for TB vaccine research.<\/jats:p>","DOI":"10.1128\/cvi.00221-14","type":"journal-article","created":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T07:53:39Z","timestamp":1402559619000},"page":"1128-1136","source":"Crossref","is-referenced-by-count":18,"title":["Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis"],"prefix":"10.1128","volume":"21","author":[{"given":"Beatriz","family":"Beltr\u00e1n-Beck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Romero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Boadella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Casal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Bezos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Mazariegos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MariPaz","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth C.","family":"Galindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"P\u00e9rez de la Lastra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Villar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseba M.","family":"Garrido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3968-3390","authenticated-orcid":false,"given":"Iker A.","family":"Sevilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Asensio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Sicilia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin P.","family":"Lyashchenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6037-5873","authenticated-orcid":false,"given":"Ram\u00f3n A.","family":"Juste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gort\u00e1zar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2014,6,11]]},"reference":[{"key":"key20180919073740_B1","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/j.1365-2907.2011.00191.x","article-title":"The status of tuberculosis in European wild mammals","volume":"42","author":"Gort\u00e1zar","year":"2012","journal-title":"Mammal Rev."},{"key":"key20180919073740_B2","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.vetmic.2011.02.041","article-title":"Progress in the control of bovine tuberculosis in Spanish wildlife","volume":"151","author":"Gort\u00e1zar","year":"2011","journal-title":"Vet. 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