{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:09:01Z","timestamp":1792714141862,"version":"4.5.0"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Having reported that pretreatment of serum samples with EDTA at 100\u00b0C improved the sensitivity for the detection of\n            <jats:italic>Histoplasma<\/jats:italic>\n            antigenemia, we have evaluated this method for the detection of\n            <jats:italic>Coccidioides<\/jats:italic>\n            antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista\n            <jats:italic>Coccidioides<\/jats:italic>\n            enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of\n            <jats:italic>Coccidioides<\/jats:italic>\n            antigenemia.\n          <\/jats:p>","DOI":"10.1128\/cvi.00227-09","type":"journal-article","created":{"date-parts":[[2009,8,13]],"date-time":"2009-08-13T02:04:37Z","timestamp":1250129077000},"page":"1453-1456","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Detection of\n            <i>Coccidioides<\/i>\n            Antigenemia following Dissociation of Immune Complexes"],"prefix":"10.1128","volume":"16","author":[{"given":"Michelle","family":"Durkin","sequence":"first","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"MiraBella Technologies, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lois","family":"Estok","sequence":"additional","affiliation":[{"name":"El Rio Special Immunology Associates, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane","family":"Hospenthal","sequence":"additional","affiliation":[{"name":"Brook Army Medical Center, Fort Sam Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Crum-Cianflone","sequence":"additional","affiliation":[{"name":"Naval Medical Center, San Diego, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Swartzentruber","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"MiraBella Technologies, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Hackett","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"MiraBella Technologies, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Joseph","family":"Wheat","sequence":"additional","affiliation":[{"name":"MiraVista Diagnostics, Indianapolis, Indiana"},{"name":"MiraBella Technologies, Indianapolis, Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00071-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"e69","DOI":"10.1086\/592073","volume":"47","year":"2008","unstructured":"Durkin, M., P. Connolly, T. Kuberski, R. Myers, B. M. Kubak, D. Bruckner, D. Pegues, and L. J. Wheat. 2008. Diagnosis of coccidioidomycosis with use of the Coccidioides antigen enzyme immunoassay. Clin. Infect. Dis.47:e69-e73.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4873-4875.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"645","DOI":"10.4269\/ajtmh.1984.33.645","volume":"33","year":"1984","unstructured":"Galgiani, J. N., K. O. Dugger, J. I. Ito, and M. A. Wieden. 1984. Antigenemia in primary coccidioidomycosis. Am. J. Trop. Med. Hyg.33:645-649.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1093\/infdis\/163.3.671","volume":"163","year":"1991","unstructured":"Galgiani, J. N., G. M. Grace, and L. L. Lundergan. 1991. New serologic tests for early detection of coccidioidomycosis. J. Infect. Dis.163:671-674.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.3.618-619.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00409-08"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.1.136-142.1983"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.ccm.2009.02.011","volume":"30","year":"2009","unstructured":"Wheat, L. J. 2009. Approach to the diagnosis of the endemic mycoses. Clin. Chest Med.30:379-389.","journal-title":"Clin. Chest Med."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00227-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00227-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T15:54:53Z","timestamp":1627574093000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00227-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":9,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1128\/CVI.00227-09"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00227-09","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10]]},"assertion":[{"value":"2009-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}