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In this study, we truncated the LigA conserved region into 7 fragments comprising the 1st to 3rd (LigACon1-3), 4th to 7.5th (LigACon4-7.5), 4th (LigACon4), 4.5th to 5.5th (LigACon4.5\u20135.5), 5.5th to 6.5th (LigACon5.5\u20136.5), 4th to 5th (LigACon4-5), and 6th to 7.5th (LigACon6-7.5) repeat domains. All 7 recombinant Lig proteins were screened using a slot-shaped dot blot assay for the diagnosis of equine leptospirosis. Our results showed that LigACon4-7.5 is the best candidate diagnostic antigen in a slot-shaped dot blot assay. LigACon4-7.5 was further evaluated as an indirect enzyme-linked immunosorbent assay (ELISA) antigen for the detection of<jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>antibodies in equine sera. This assay was evaluated with equine sera (<jats:italic>n<\/jats:italic>= 60) that were microscopic agglutination test (MAT) negative and sera (<jats:italic>n<\/jats:italic>= 220) that were MAT positive to the 5 serovars that most commonly cause equine leptospirosis. The indirect ELISA results showed that at a single serum dilution of 1:250, the sensitivity and specificity of ELISA were 80.0% and 87.2%, respectively, compared to those of MAT. In conclusion, an indirect ELISA was developed utilizing a recombinant LigA fragment comprising the 4th to 7.5th repeat domain (LigACon4-7.5) as a diagnostic antigen for equine leptospirosis. This ELISA was found to be sensitive and specific, and it yielded results that concurred with those of the standard MAT.<\/jats:p>","DOI":"10.1128\/cvi.00245-13","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T04:47:30Z","timestamp":1369889250000},"page":"1143-1149","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Development of an Enzyme-Linked Immunosorbent Assay Using a Recombinant LigA Fragment Comprising Repeat Domains 4 to 7.5 as an Antigen for Diagnosis of Equine Leptospirosis"],"prefix":"10.1128","volume":"20","author":[{"given":"Weiwei","family":"Yan","sequence":"first","affiliation":[{"name":"Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Hassan","family":"Saleem","sequence":"additional","affiliation":[{"name":"Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"McDonough","sequence":"additional","affiliation":[{"name":"Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"McDonough","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Divers","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Fu","family":"Chang","sequence":"additional","affiliation":[{"name":"Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2208"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1097\/QCO.0b013e32814a5729","article-title":"Leptospirosis: pathogenesis, immunity, and diagnosis","volume":"20","author":"Palaniappan RU","year":"2007","unstructured":"PalaniappanRU RamanujamS ChangYF. 2007. 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