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It is believed that vaccines inducing anti-adhesin immunity to inhibit bacterial adherence and anti-toxin immunity to eliminate toxin activity would provide broad-spectrum protection against ETEC. In this study, an ETEC fimbrial adhesin was used as a platform to express LT and STa for adhesin-toxin fusion antigens to induce anti-toxin and anti-adhesin immunity. An epitope from the B subunit of LT toxin (LTP1,<jats:sup>8<\/jats:sup>LCSEYRNTQIYTIN<jats:sup>21<\/jats:sup>) and an STa toxoid epitope (<jats:sup>5<\/jats:sup>CCELCCNPQCAGCY<jats:sup>18<\/jats:sup>) were embedded in the FaeG major subunit of<jats:italic>E. coli<\/jats:italic>K88ac fimbriae. Constructed K88ac-toxin chimeric fimbriae were harvested and used for rabbit immunization. Immunized rabbits developed anti-K88ac, anti-LT, and anti-STa antibodies. Moreover, induced antibodies not only inhibited adherence of K88ac fimbrial<jats:italic>E. coli<\/jats:italic>to porcine small intestinal enterocytes but also neutralized cholera toxin and STa toxin. Data from this study demonstrated that K88ac fimbriae expressing LT and STa epitope antigens elicited neutralizing anti-toxin antibodies and anti-adhesin antibodies and suggested that<jats:italic>E. coli<\/jats:italic>fimbriae could serve as a platform for the development of broad-spectrum vaccines against ETEC.<\/jats:p>","DOI":"10.1128\/cvi.00251-10","type":"journal-article","created":{"date-parts":[[2010,10,28]],"date-time":"2010-10-28T03:11:13Z","timestamp":1288235473000},"page":"1859-1867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["<i>Escherichia coli<\/i>K88ac Fimbriae Expressing Heat-Labile and Heat-Stable (STa) Toxin Epitopes Elicit Antibodies That Neutralize Cholera Toxin and STa Toxin and Inhibit Adherence of K88ac Fimbrial<i>E. coli<\/i>"],"prefix":"10.1128","volume":"17","author":[{"given":"Chengxian","family":"Zhang","sequence":"first","affiliation":[{"name":"Veterinary and Biomedical Sciences Department, The Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Veterinary and Biomedical Sciences Department, The Center for Infectious Disease Research and Vaccinology, South Dakota State University, Brookings, South Dakota 57007"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"151","year":"1994","unstructured":"Alexander, T. 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