{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:46:54Z","timestamp":1791323214674,"version":"4.3.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            While it is well established that proximity to wetlands is a risk factor for contracting Buruli ulcer, it is not clear what proportion of a population living in an area where the etiologic agent,\n            <jats:italic>Mycobacterium ulcerans<\/jats:italic>\n            , is endemic is actually exposed to this disease. Immunological cross-reactivity among mycobacterial species complicates the development of a specific serological test. Among immunodominant proteins recognized by a panel of anti-\n            <jats:italic>M. ulcerans<\/jats:italic>\n            monoclonal antibodies, the\n            <jats:italic>M. ulcerans<\/jats:italic>\n            homologue of the\n            <jats:italic>M. leprae<\/jats:italic>\n            18-kDa small heat shock protein (shsp) was identified. Since this shsp has no homologues in\n            <jats:italic>M. bovis<\/jats:italic>\n            and\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            , we evaluated its use as a target antigen for a serological test. Anti-18-kDa shsp antibodies were frequently found in the sera of Buruli ulcer patients and of healthy household contacts but rarely found in controls from regions where the infection is not endemic. The results indicate that only a small proportion of\n            <jats:italic>M. ulcerans<\/jats:italic>\n            -infected individuals contract the clinical disease.\n          <\/jats:p>","DOI":"10.1128\/cvi.00254-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:38:08Z","timestamp":1165430288000},"page":"1314-1321","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Use of the Immunodominant 18-Kilodalton Small Heat Shock Protein as a Serological Marker for Exposure to\n            <i>Mycobacterium ulcerans<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Diana","family":"Diaz","sequence":"first","affiliation":[{"name":"Molecular Immunology, Swiss Tropical Institute, CH-4002 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"Do\u0308beli","sequence":"additional","affiliation":[{"name":"F. Hoffmann La-Roche, Ltd., CH-4070 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy","family":"Yeboah-Manu","sequence":"additional","affiliation":[{"name":"Molecular Immunology, Swiss Tropical Institute, CH-4002 Basel, Switzerland"},{"name":"Noguchi Memorial Institute for Medical Research, Legon, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernestina","family":"Mensah-Quainoo","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Tema District, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arno","family":"Friedlein","sequence":"additional","affiliation":[{"name":"F. Hoffmann La-Roche, Ltd., CH-4070 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Soder","sequence":"additional","affiliation":[{"name":"F. Hoffmann La-Roche, Ltd., CH-4070 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Rondini","sequence":"additional","affiliation":[{"name":"Molecular Immunology, Swiss Tropical Institute, CH-4002 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bodmer","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Pluschke","sequence":"additional","affiliation":[{"name":"Molecular Immunology, Swiss Tropical Institute, CH-4002 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.4269\/ajtmh.1998.59.1015","volume":"59","year":"1998","unstructured":"Asiedu, K., and S. Etuaful. 1998. 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