{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T21:26:52Z","timestamp":1770672412435,"version":"3.49.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Trichinellosis is a zoonotic disease caused by the consumption of raw or semiraw meat from different animals harboring\n            <jats:italic>Trichinella<\/jats:italic>\n            larvae in their muscles. Since there are no pathognomonic signs, diagnosis can be difficult; for this reason, serology is important. The objective of this study was to validate an enzyme-linked immunosorbent assay (ELISA) using excretory\/secretory antigens to detect anti-\n            <jats:italic>Trichinella<\/jats:italic>\n            immunoglobulin G antibodies in human sera. A total of 3,505 human serum samples were tested. A receiver-operator characteristic (ROC) curve analysis was performed. The accuracy of the test was determined by calculating the area under the curve, which was equal to 0.999, indicating high accuracy. The coefficient of variation calculated for data from four serum samples in eight working sessions was no higher than 5% for the positive sera or 14% for the negative sera. Moreover, the analysis of the differences in optical density between duplicates indicated a high repeatability for the ELISA. At the ROC optimized cutoff, the sensitivity and specificity of the test were, respectively, 99.2% and 90.6% (specificity of 95.6% when excluding the samples from multiparasitized persons from Tanzania). The validated ELISA showed good performance in terms of sensitivity, repeatability, and reproducibility, whereas the specificity was limited. These results suggest that this test is suitable for detecting anti-\n            <jats:italic>Trichinella<\/jats:italic>\n            antibodies in human sera for diagnostic purposes, whereas its use in epidemiological surveys could be questionable.\n          <\/jats:p>","DOI":"10.1128\/cvi.00257-08","type":"journal-article","created":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T03:29:11Z","timestamp":1222831751000},"page":"1723-1729","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Validation of an Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Trichinellosis"],"prefix":"10.1128","volume":"15","author":[{"given":"Maria Angeles","family":"Go\u0301mez-Morales","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Ludovisi","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Amati","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Cherchi","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizio","family":"Pezzotti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edoardo","family":"Pozio","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita\u0300"},{"name":"Agenzia di Sanita\u0300 Pubblica, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"325","volume":"39","year":"2005","unstructured":"Akisu, C., S. 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