{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:33:39Z","timestamp":1761597219086},"reference-count":24,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To date, the most promising vaccination strategies for the control of bovine tuberculosis (TB) focus on improving the efficacy of\n            <jats:italic>M<\/jats:italic>\n            <jats:italic>ycobacterium<\/jats:italic>\n            <jats:italic>bovis<\/jats:italic>\n            bacillus Calmette-Gue\u0301rin (BCG). However, vaccination with BCG results in sensitization of animals to bovine tuberculin and compromises tests currently used for diagnosis of bovine TB infection. Thus, the development of specific diagnostic reagents capable of discriminating between infected and uninfected vaccinated animals (DIVA) is of high priority. To test the hypothesis that\n            <jats:italic>M. bovis<\/jats:italic>\n            -secreted proteins are likely to contain immunogenic antigens that can be used to increase the specificity of diagnostic tests, we screened 379 pools of overlapping peptides representing 119 antigens for their ability to stimulate a gamma inferferon (IFN-\u03b3) response\n            <jats:italic>in vitro<\/jats:italic>\n            using whole blood from both TB reactor and BCG-vaccinated animals. Peptide pools representing antigens Rv3020c and Rv2346c induced responses in 61% and 57% of the TB reactor animals, respectively, without inducing responses in any BCG-vaccinated animal studied. Furthermore, individual peptides contained within pools recognized by BCG vaccinates were identified that were specific and induced IFN-\u03b3 responses in TB reactor animals. From these results, we constructed a cocktail of nine peptides representing multiple antigen targets that was recognized by 54% of TB reactor animals but also failed to induce responses in any BCG-vaccinated animal studied. In summary, we have identified three peptide cocktails for prioritization in larger trials to discriminate between\n            <jats:italic>M. bovis<\/jats:italic>\n            infection and BCG vaccination.\n          <\/jats:p>","DOI":"10.1128\/cvi.00261-10","type":"journal-article","created":{"date-parts":[[2010,7,29]],"date-time":"2010-07-29T06:25:47Z","timestamp":1280384747000},"page":"1344-1348","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Screening of Predicted Secreted Antigens from\n            <i>Mycobacterium bovis<\/i>\n            Identifies Potential Novel Differential Diagnostic Reagents"],"prefix":"10.1128","volume":"17","author":[{"given":"Gareth J.","family":"Jones","sequence":"first","affiliation":[{"name":"TB Research Group, Veterinary Laboratories Agency\u2014Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Glyn","family":"Hewinson","sequence":"additional","affiliation":[{"name":"TB Research Group, Veterinary Laboratories Agency\u2014Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Martin","family":"Vordermeier","sequence":"additional","affiliation":[{"name":"TB Research Group, Veterinary Laboratories Agency\u2014Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01184-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3719-3728.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5419.1520"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S0007-1935(17)31792-X","volume":"137","year":"1981","unstructured":"Berggren, S. A. 1981. Field experiment with BCG vaccine in Malawi. Br. Vet. J.137:88-96.","journal-title":"Br. Vet. J."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1073\/pnas.052548299","volume":"99","year":"2002","unstructured":"Brosch, R., S. V. Gordon, M. Marmiesse, P. Brodin, C. Buchrieser, K. Eiglmeier, T. Garnier, C. Gutierrez, G. Hewinson, K. Kremer, L. M. Parsons, A. S. Pym, S. Samper, D. van Soolingen, and S. T. Cole. 2002. A new evolutionary scenario for the Mycobacterium tuberculosis complex. Proc. Natl. Acad. Sci. U. S. A.99:3684-3689.","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/0034-5288(95)90023-3","volume":"59","year":"1995","unstructured":"Buddle, B. M., D. Keen, A. Thomson, G. Jowett, A. R. McCarthy, J. Heslop, G. W. De Lisle, J. L. Stanford, and F. E. Aldwell. 1995. 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Human Mycobacterium bovis infection in the United Kingdom: incidence, risks, control measures and review of the zoonotic aspects of bovine tuberculosis. Tuberculosis (Edinb.)86:77-109.","journal-title":"Tuberculosis (Edinb.)"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1046\/j.1365-2958.1999.01383.x","volume":"32","year":"1999","unstructured":"Gordon, S. V., R. Brosch, A. Billault, T. Garnier, K. Eiglmeier, and S. T. Cole. 1999. Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays. Mol. Microbiol.32:643-655.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01246-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1086\/318081","volume":"183","year":"2001","unstructured":"Lalvani, A., P. Nagvenkar, Z. Udwadia, A. A. Pathan, K. A. Wilkinson, J. S. Shastri, K. Ewer, A. V. Hill, A. Mehta, and C. Rodrigues. 2001. Enumeration of T cells specific for RD1-encoded antigens suggests a high prevalence of latent Mycobacterium tuberculosis infection in healthy urban Indians. J. Infect. Dis.183:469-477.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1365-2567.2006.02355.x","volume":"118","year":"2006","unstructured":"Rogerson, B. J., Y. J. Jung, R. LaCourse, L. Ryan, N. Enright, and R. J. North. 2006. Expression levels of Mycobacterium tuberculosis antigen-encoding genes versus production levels of antigen-specific T cells during stationary level lung infection in mice. Immunology118:195-201.","journal-title":"Immunology"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00150-08"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"R265","DOI":"10.1186\/gb-2007-8-12-r265","volume":"8","year":"2007","unstructured":"Sidders, B., M. Withers, S. L. Kendall, J. Bacon, S. J. 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