{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:41:36Z","timestamp":1780670496041,"version":"3.54.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Detection of antibody to C\n            <jats:sub>6<\/jats:sub>\n            , a peptide that reproduces the sequence of the sixth invariable region within the central domain of the VlsE protein of\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , is used currently for the serologic diagnosis of Lyme disease in humans.\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            isolates taken from infected humans can be categorized into specific genetic subtypes (designated RST1, -2, and -3) by restriction fragment length polymorphisms in the 16S to 23S rRNA spacer sequence. Many of these, usually categorized as RST2, retain only segments of the linear plasmid lp28-1, which encodes VlsE. The VlsE genetic region is retained, but altered expression of this molecule could affect diagnosis by the C\n            <jats:sub>6<\/jats:sub>\n            enzyme-linked immunosorbent assay (ELISA). Serum samples from patients infected with each of the three genotypes and from mice infected with three RST2 isolates were tested with the C\n            <jats:sub>6<\/jats:sub>\n            ELISA. Such isolates elicited marked C\n            <jats:sub>6<\/jats:sub>\n            responses in infected mice. The sensitivity of C\n            <jats:sub>6<\/jats:sub>\n            antibody detection in patients infected with RST2 spirochetes was statistically indistinguishable from detection of RST1 and RST3 infections. These findings demonstrate that diagnosis by C\n            <jats:sub>6<\/jats:sub>\n            ELISA remains effective for infection with all\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            genotypes, including those with incomplete lp28-1 plasmids.\n          <\/jats:p>","DOI":"10.1128\/cvi.00266-06","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T04:24:04Z","timestamp":1163651044000},"page":"90-93","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["<i>Borrelia burgdorferi<\/i>\n            Spirochetes That Harbor Only a Portion of the lp28-1 Plasmid Elicit Antibody Responses Detectable with the C\n            <sub>6<\/sub>\n            Test for Lyme Disease"],"prefix":"10.1128","volume":"14","author":[{"given":"Monica E.","family":"Embers","sequence":"first","affiliation":[{"name":"Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology &amp; Immunology, New York Medical College, Valhalla, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary P.","family":"Wormser","sequence":"additional","affiliation":[{"name":"Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology &amp; Immunology, New York Medical College, Valhalla, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology &amp; Immunology, New York Medical College, Valhalla, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale S.","family":"Martin","sequence":"additional","affiliation":[{"name":"Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology &amp; Immunology, New York Medical College, Valhalla, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario T.","family":"Philipp","sequence":"additional","affiliation":[{"name":"Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana"},{"name":"Division of Infectious Diseases, Department of Medicine"},{"name":"Department of Microbiology &amp; Immunology, New York Medical College, Valhalla, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1086\/374395","volume":"187","year":"2003","unstructured":"Bacon, R. 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