{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:26Z","timestamp":1787016626382,"version":"build-2736575974"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Responsible for 9 million new cases of active disease and nearly 2 million deaths each year, tuberculosis (TB) remains a global health threat of overwhelming dimensions.\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>\n                    BCG, the only licensed vaccine available, fails to confer lifelong protection and to prevent reactivation of latent infection. Although 15 new vaccine candidates are now in clinical trials, an effective vaccine against TB remains elusive, and new strategies for vaccination are vital. BCG vaccination fails to induce immunity against\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    latency antigens. Synthetic long peptides (SLPs) combined with adjuvants have been studied mostly for therapeutic cancer vaccines, yet not for TB, and proved to induce efficient antitumor immunity. This study investigated an SLP derived from Rv1733c, a major\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    latency antigen which is highly expressed by \u201cdormant\u201d\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    and well recognized by T cells from latently\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    -infected individuals. In order to assess its\n                    <jats:italic>in vivo<\/jats:italic>\n                    immunogenicity and protective capacity, Rv1733c SLP in CpG was administered to HLA-DR3 transgenic mice. Immunization with Rv1733c SLP elicited gamma interferon-positive\/tumor necrosis factor-positive (IFN-\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    \/TNF\n                    <jats:sup>+<\/jats:sup>\n                    ) and IFN-\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells and Rv1733c-specific antibodies and led to a significant reduction in the bacterial load in the lungs of\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    -challenged mice. This was observed both in a pre- and in a post-\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    challenge setting. Moreover, Rv1733c SLP immunization significantly boosted the protective efficacy of BCG, demonstrating the potential of\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    latency antigens to improve BCG efficacy. These data suggest a promising role for\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    latency antigen Rv1733c-derived SLPs as a novel TB vaccine approach, both in a prophylactic and in a postinfection setting.\n                  <\/jats:p>","DOI":"10.1128\/cvi.00271-15","type":"journal-article","created":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T21:12:34Z","timestamp":1437599554000},"page":"1060-1069","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Synthetic Long Peptide Derived from Mycobacterium tuberculosis Latency Antigen Rv1733c Protects against Tuberculosis"],"prefix":"10.1128","volume":"22","author":[{"given":"Mariateresa","family":"Coppola","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J. F.","family":"van den Eeden","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kees L. M. C.","family":"Franken","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom H. M.","family":"Ottenhoff","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemieke","family":"Geluk","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global tuberculosis report 2014","author":"World Health Organization","year":"2014","unstructured":"World Health Organization. 2014. Global tuberculosis report 2014. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_3_2","volume-title":"The global plan to stop TB 2011\u20132015","author":"Stop TB Partnership","year":"2011","unstructured":"Stop TB Partnership. 2011. The global plan to stop TB 2011\u20132015. Stop TB Partnership, Geneva, Switzerland."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002607"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030205"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01137-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.03.018"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2011.12.013"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2174\/187153008783928398"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00111-09"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141602"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2011.12.005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2285"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46252"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.07.144"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1880"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2373"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24597"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1881"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343324"},{"key":"e_1_3_2_21_2","first-page":"105","article-title":"HLA class II restriction repertoire of antigen-specific T cells. I","volume":"13","author":"Ottenhoff TH","year":"1985","unstructured":"Ottenhoff TH, Elferink DG, Hermans J, de Vries RR. 1985. HLA class II restriction repertoire of antigen-specific T cells. I. The main restriction determinants for antigen presentation are associated with HLA-D\/DR and not with DP and DQ. Hum Immunol 13:105\u2013116.","journal-title":"The main restriction determinants for antigen presentation are associated with HLA-D\/DR and not with DP and DQ. Hum Immunol"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1991.tb00828.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.11.6463"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10797"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100980"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1999.1162"},{"key":"e_1_3_2_27_2","first-page":"104","article-title":"Negative and positive selection by HLA-DR3(DRw17) molecules in transgenic mice","volume":"40","author":"Strauss G","year":"1994","unstructured":"Strauss G, Vignali DA, Schonrich G, Hammerling GJ. 1994. Negative and positive selection by HLA-DR3(DRw17) molecules in transgenic mice. Immunogenetics 40:104\u2013108.","journal-title":"Immunogenetics"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90448-8"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.3.1167"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.27"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.4049\/jimmunol.156.7.2473","article-title":"Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules","volume":"156","author":"Newberg MH","year":"1996","unstructured":"Newberg MH, Smith DH, Haertel SB, Vining DR, Lacy E, Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156:2473\u20132480.","journal-title":"J Immunol"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.147-152.2002"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.6.3973"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270406"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707331104"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1592"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300248"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2299"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040455"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838693"},{"key":"e_1_3_2_41_2","first-page":"1049","article-title":"Spontaneous cytokine production and its effect on induced production","volume":"9","author":"Walker D","year":"2002","unstructured":"Walker D, Jason J, Wallace K, Slaughter J, Whatley V, Han A, Nwanyanwu OC, Kazembe PN, Dobbie H, Archibald L, Jarvis WR. 2002. Spontaneous cytokine production and its effect on induced production. Clin Diagn Lab Immunol 9:1049\u20131056.","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.02.009"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.10.045"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.05.005"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00256"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/31159"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903299"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-199588"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit281"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1243-1"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-396548-6.00004-4"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir548"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01999-06"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01990-06"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027477"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901720106"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/22326"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020404"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis425"},{"key":"e_1_3_2_60_2","doi-asserted-by":"crossref","first-page":"2864","DOI":"10.4049\/jimmunol.149.9.2864","article-title":"Functional analysis of DR17(DR3)-restricted mycobacterial T cell epitopes reveals DR17-binding motif and enables the design of allele-specific competitor peptides","volume":"149","author":"Geluk A","year":"1992","unstructured":"Geluk A, van Meijgaarden KE, Janson AA, Drijfhout JW, Meloen RH, de Vries RR, Ottenhoff TH. 1992. Functional analysis of DR17(DR3)-restricted mycobacterial T cell epitopes reveals DR17-binding motif and enables the design of allele-specific competitor peptides. J Immunol 149:2864\u20132871.","journal-title":"J Immunol"}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00271-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00271-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T08:21:22Z","timestamp":1691828482000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00271-15"}},"subtitle":[],"editor":[{"given":"D. W.","family":"Pascual","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":59,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1128\/CVI.00271-15"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00271-15","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"2015-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}