{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:12:51Z","timestamp":1761894771298},"reference-count":11,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A total of 20 patients with alveolar echinococcosis in different clinical stages according to the WHO-PNM staging system (P, parasitic mass in the liver; N, involvement of neighboring organs; M, metastasis) were followed up serologically with the commercial\n            <jats:italic>Echinococcus<\/jats:italic>\n            Western Blot IgG assay and a crude antigen extract enzyme-linked immunosorbent assay (ELISA). The cohort included patients after curative resection and patients who had unresectable lesions with stable disease or progressive infection. There were visible correlations of the crude antigen ELISA index and the presence and intensity of diagnostic bands in the Western blot. In most patients after curative resection, bands at 7, 16, and 18 kDa markedly decreased or vanished after 1 to 4 years. In a patient with a nonviable lesion (it died out), bands at 16 and 18 kDa vanished after 4 years. Among individuals with unresectable lesions but stable disease under antiparasitic chemotherapy, a decrease of all diagnostic bands was visible after 2 to 3 years in half of the patients, whereas the other half had unchanged blot results after 4 to 6 years. Patients with progressive disease showed increasing intensities of bands at 16, 18, and 7 kDa. The change of banding patterns was not influenced by the PNM stage in patients after curative surgery or with unresectable lesions. Our data indicate a correlation of the 7-, 16-, and 18-kDa-Western blot bands with disease activity independent of the PNM stage. This study demonstrated the usefulness of the\n            <jats:italic>Echinococcus<\/jats:italic>\n            Western Blot IgG assay as an additional serological test for the follow-up of patients with alveolar echinococcosis.\n          <\/jats:p>","DOI":"10.1128\/cvi.00272-08","type":"journal-article","created":{"date-parts":[[2008,9,18]],"date-time":"2008-09-18T00:54:29Z","timestamp":1221699269000},"page":"1633-1637","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of a Commercial\n            <i>Echinococcus<\/i>\n            Western Blot Assay for Serological Follow-Up of Patients with Alveolar Echinococcosis"],"prefix":"10.1128","volume":"15","author":[{"given":"Dennis","family":"Tappe","sequence":"first","affiliation":[{"name":"Institute of Hygiene and Microbiology, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"},{"name":"Comprehensive Infectious Diseases Center, Division of Infectiology and Clinical Immunology, University Hospital and Medical Center Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate","family":"Gru\u0308ner","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Microbiology, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"},{"name":"Comprehensive Infectious Diseases Center, Division of Infectiology and Clinical Immunology, University Hospital and Medical Center Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Kern","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Microbiology, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"},{"name":"Comprehensive Infectious Diseases Center, Division of Infectiology and Clinical Immunology, University Hospital and Medical Center Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Frosch","sequence":"additional","affiliation":[{"name":"Institute of Hygiene and Microbiology, University of Wu\u0308rzburg, Wu\u0308rzburg, Germany"},{"name":"Comprehensive Infectious Diseases Center, Division of Infectiology and Clinical Immunology, University Hospital and Medical Center Ulm, Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1046\/j.1365-3156.1997.d01-385.x","volume":"2","year":"1997","unstructured":"Dreweck, C. M., C. G. L\u00fcder, P. T. Soboslay, and P. Kern. 1997. Subclass-specific serological reactivity and IgG4-specific antigen recognition in human echinococcosis. Trop. Med. Int. Health2:779-787.","journal-title":"Trop. Med. Int. Health"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1007\/s00535-004-1559-7","volume":"40","year":"2005","unstructured":"Fujimoto, Y., A. Ito, Y. Ishikawa, M. Inoue, Y. Suzuki, M. Ohhira, T. Ohtake, and Y. Kohgo. 2005. Usefulness of recombinant Em18-ELISA to evaluate efficacy of treatment in patients with alveolar echinococcosis. J. Gastroenterol.40:426-431.","journal-title":"J. Gastroenterol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0035-9203(89)90512-9","volume":"83","year":"1989","unstructured":"Gottstein, B., K. Tschudi, J. Eckert, and R. Ammann. 1989. Em2-ELISA for the follow-up of alveolar echinococcosis after complete surgical resection of liver lesions. Trans. R. Soc. Trop. Med. Hyg.83:389-393.","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/0035-9203(93)90476-7","volume":"87","year":"1993","unstructured":"Ito, A., M. Nakao, H. Kutsumi, M. W. Lightowlers, M. Itoh, and S. Sato. 1993. Serodiagnosis of alveolar hydatid disease by Western blotting. Trans. R. Soc. Trop. Med. Hyg.87:170-172.","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.4269\/ajtmh.1995.52.41","volume":"52","year":"1995","unstructured":"Ito, A., P. M. Schantz, and J. F. Wilson. 1995. Em18, a new serodiagnostic marker for differentiation of active and inactive cases of alveolar hydatid disease. Am. J. Trop. Med. Hyg.52:41-44.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"S283","DOI":"10.1016\/j.parint.2005.11.041","volume":"55","year":"2006","unstructured":"Kern, P., H. Wen, N. Sato, D. A. Vuitton, B. Gruener, Y. Shao, E. Delabrousse, W. Kratzer, and S. Bresson-Hadni. 2006. WHO classification of alveolar echinococcosis: principles and application. Parasitol. Int.55 (Suppl.):S283-S287.","journal-title":"Parasitol. Int."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3718-3721.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/S0035-9203(97)90291-1","volume":"91","year":"1997","unstructured":"Ma, L., A. Ito, Y. H. Liu, X. G. Wang, Y. Q. Yao, D. G. Yu, and Y. T. Chen. 1997. Alveolar echinococcosis: Em2plus-ELISA and Em18-Western blots for follow-up after treatment with albendazole. Trans. R. Soc. Trop. Med. Hyg.91:476-478.","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"e_1_3_2_10_2","first-page":"171","volume":"100","year":"2007","unstructured":"Makni, F., L. Hachicha, F. Mseddi, H. Hammami, F. Cheikhrouhou, H. Sellami, A. Sellami, R. Mzali, S. Boujelb\u00e8ne, R. Reba\u00ef, I. Beyrouti, and A. Ayadi. 2007. Contribution of Western blotting to the diagnosis of hydatidosis. French. Bull. Soc. Pathol. Exot.100:171-173.","journal-title":"French. Bull. Soc. Pathol. Exot."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.4269\/ajtmh.1983.32.1381","volume":"32","year":"1983","unstructured":"Schantz, P. M., J. F. Wilson, S. P. Wahlquist, L. P. Boss, and R. L. Rausch. 1983. Serologic tests for diagnosis and post-treatment evaluation of patients with alveolar hydatid disease (Echinococcus multilocularis). Am. J. Trop. Med. Hyg.32:1381-1386.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/0035-9203(95)90449-2","volume":"89","year":"1995","unstructured":"Wen, H., S. Bresson-Hadni, D. A. Vuitton, D. Lenys, B. M. Yang, Z. X. Ding, and P. S. Craig. 1995. Analysis of immunoglobulin G subclass in the serum antibody responses of alveolar echinococcosis patients after surgical treatment and chemotherapy as an aid to assessing the outcome. Trans. R. Soc. Trop. Med. Hyg.89:692-697.","journal-title":"Trans. R. Soc. Trop. Med. Hyg."}],"container-title":["Clinical and Vaccine Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00272-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/CVI.00272-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T15:54:28Z","timestamp":1627574068000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/CVI.00272-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11]]},"references-count":11,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1128\/CVI.00272-08"],"URL":"https:\/\/doi.org\/10.1128\/cvi.00272-08","relation":{},"ISSN":["1556-6811","1556-679X"],"issn-type":[{"value":"1556-6811","type":"print"},{"value":"1556-679X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11]]},"assertion":[{"value":"2008-07-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}