{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T22:12:24Z","timestamp":1761948744811,"version":"build-2065373602"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n            , a self-replicating cell wall-deficient prokaryote, has a differentiated terminal organelle that is essential for cytadherence and gliding motility. P30, an important protein associated with the terminal organelle, is required for the cytadherence and virulence of\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            . P30 is a transmembrane protein with an intracytoplasmic N terminus and an exposed C terminus. In the present study, we amplified and sequenced the full-length\n            <jats:italic>p30<\/jats:italic>\n            gene of\n            <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n            directly from 18 Indian asthmatic patients. Sequence diversity was observed in the\n            <jats:italic>p30<\/jats:italic>\n            genes from 16 clinical samples when the sequences were compared with the sequence of strain M-129. We also successfully expressed a fragment of the\n            <jats:italic>p30<\/jats:italic>\n            gene (P30B) that includes the complete C-terminal proline-rich amino acid sequences in different\n            <jats:italic>Escherichia coli<\/jats:italic>\n            expression systems. The maltose binding protein (MBP)-P30B fusion protein was recognized by\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            -infected patient sera in immunoblots, and the protein was immunogenic in mice. We further analyzed the reactivity of the MBP-P30B fusion protein with patient sera in an enzyme-linked immunosorbent assay (ELISA) and compared it with the reactivity obtained with a commercial kit (the Serion ELISA Classic kit). The sensitivity and the specificity of the in-house ELISA were 78.57% and 89.47%, respectively. This study suggests that the P30 protein can be used as an antigen along with other adhesin proteins for the immunodiagnosis of\n            <jats:italic>M. pneumoniae<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/cvi.00283-07","type":"journal-article","created":{"date-parts":[[2007,11,22]],"date-time":"2007-11-22T02:06:34Z","timestamp":1195697194000},"page":"215-220","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Cloning, Expression, and Immunological Characterization of the P30 Protein of\n            <i>Mycoplasma pneumoniae<\/i>"],"prefix":"10.1128","volume":"15","author":[{"given":"Avanish Kumar","family":"Varshney","sequence":"first","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"},{"name":"Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India"},{"name":"International Center for Genetic Engineering and Biotechnology, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rama","family":"Chaudhry","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"},{"name":"Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India"},{"name":"International Center for Genetic Engineering and Biotechnology, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushil Kumar","family":"Kabra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"},{"name":"Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India"},{"name":"International Center for Genetic Engineering and Biotechnology, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawan","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India"},{"name":"Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India"},{"name":"International Center for Genetic Engineering and Biotechnology, New Delhi, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1136\/thorax.58.1.1","volume":"58","year":"2003","unstructured":"Anonymous. 2003. 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