{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:15:57Z","timestamp":1777526157992,"version":"3.51.4"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Clin Vaccine Immunol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Paracoccidioidomycosis (PCM) is a granulomatous disease caused by the dimorphic fungus\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            . The immunoglobulin classes and isotypes of antibodies directed to acidic glycosphingolipids (GSLs) and glucosylceramide of\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            were determined by enzyme-linked immunosorbent assay of sera from 31 PCM patients. The reactivities of 38 serum samples were analyzed by considering the stage of treatment: before antifungal treatment (\n            <jats:italic>n<\/jats:italic>\n            = 10), during 1 to 4 months of treatment (T1-4;\n            <jats:italic>n<\/jats:italic>\n            = 9), during 5 to 12 months of treatment (T5-12;\n            <jats:italic>n<\/jats:italic>\n            = 9), and posttreatment (PT;\n            <jats:italic>n<\/jats:italic>\n            = 10). Sera from healthy subjects (\n            <jats:italic>n<\/jats:italic>\n            = 12) were used as controls. Only the GSL Pb-1 antigen, which presents the carbohydrate structure Gal\n            <jats:italic>f<\/jats:italic>\n            \u03b21-6(Man\u03b11-3)Man\u03b21, was reactive with the PCM patient sera. The PCM patient sera did not react with Pb-2, which lacks the Gal\n            <jats:italic>f<\/jats:italic>\n            residue and which is considered the biosynthetic precursor of Pb-1, indicating that the Gal\n            <jats:italic>f<\/jats:italic>\n            residue is essential for antibody reactivity. The Pb-1 glycolipid from nontreated patients elicited a primary immune response with immunoglobulin M (IgM) production and subsequent switching to IgG1 production. The IgG1 titer increased after the start of antifungal treatment (T1-4 group), and general decreases in the anti-Pb-1 antibody titers were observed after 5 months of treatment (T5-12 and PT groups). The Pb-1 antigen, an acidic GSL with terminal Gal\n            <jats:italic>f<\/jats:italic>\n            residue, has potential application as an elicitor of the host immune response in patients with PCM.\n          <\/jats:p>","DOI":"10.1128\/cvi.00285-06","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T02:04:55Z","timestamp":1164852295000},"page":"150-156","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Expression of Antibodies Directed to\n            <i>Paracoccidioides brasiliensis<\/i>\n            Glycosphingolipids during the Course of Paracoccidioidomycosis Treatment"],"prefix":"10.1128","volume":"14","author":[{"given":"Silvio","family":"Bertini","sequence":"first","affiliation":[{"name":"Department of Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaldo L.","family":"Colombo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Universidade Federal de Sa\u0303o Paulo\/Escola Paulista de Medicina, Rua Botucatu 862, Sa\u0303o Paulo, SP 04023-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helio K.","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita H.","family":"Straus","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S1286-4579(99)80022-7","volume":"1","year":"1999","unstructured":"Baida, H., P. 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